PXD050989 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Conserved helical motifs in the Ikaros IDR mediate NuRD interaction and transcriptional repression |
Description | The transcription factor IKZF1/Ikaros is essential for B cell development, and recurrently mutated in human B-ALL. Ikaros has been ascribed both activating and repressive functions via interactions with coactivator and corepressor complexes, but the relative abundance of Ikaros-associated coregulatory complexes and their contribution to Ikaros-mediated gene regulation are not well understood. To address this issue, we performed an unbiased identification of Ikaros-interacting proteins in pre-B cells, and found that Ikaros interacts overwhelmingly with corepressors and heterochromatin-associated proteins. Time-resolved analysis of transcription and chromatin state identified transcriptional repression as the immediate response to Ikaros induction. Transcriptional repression preceded transcriptional activation by several hours, and was accompanied by a rapid loss of chromatin accessibility and reduced levels of H3K27ac particularly at enhancers. Functional characterisation of intrinsically disordered regions in the Ikaros protein identified highly conserved helical motifs that mediate Ikaros association with the NuRD corepressor complex and contribute to the silencing of target genes in pre-B cells and antiproliferative functions of Ikaros in human B-ALL |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-23 |
AnnouncementXML | Submission_2024-10-23_07:49:45.963.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Alex Montoya |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | 2-pyrrolidone-5-carboxylic acid (Gln); acetylated residue; monohydroxylated residue; deamidated residue |
Instrument | Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-03-26 08:55:20 | ID requested | |
⏵ 1 | 2024-10-23 07:49:46 | announced | |
Publication List
Keyword List
submitter keyword: NuRD, corepressors,Ikaros, ikzf1, coactivators, chromatin |
Contact List
Dr Pavel Shliaha |
contact affiliation | Head Proteomics and Metabolomics Core Facility |
contact email | pavel.shliaha@lms.mrc.ac.uk |
lab head | |
Alex Montoya |
contact affiliation | Medical Research Council - London Institute of Medical Sciences |
contact email | alex.montoya@lms.mrc.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD050989
- Label: PRIDE project
- Name: Conserved helical motifs in the Ikaros IDR mediate NuRD interaction and transcriptional repression