PXD050980-1

PXD050980 is an original dataset announced via ProteomeXchange.

Title	Resting skeletal muscle reduces ATP turnover during hibernation
Description	In mammals, loss of food intake and reduced mechanical loading/activity of skeletal muscles leads to a very rapid loss in mass and function. However, during hibernation in bears, despite spending months without feeding and with very modest muscle activity, only moderate muscle wasting is observed. Part of this tissue sparing is due to a highly reduced metabolic activity in almost all tissues, including skeletal muscle. Interestingly, myosin, one of the most abundant proteins in skeletal muscle, can have different metabolic activities in inactive muscle. Therefore, to evaluate the functional and metabolic alterations in hibernating muscles, we performed an analysis on a single muscle fiber level. Individual fibers were taken from biopsies of the same bears either during hibernation or during the active phase in the summer. We confirm that muscle fibers from hibernating bears show no loss of fiber size and a mild reduction in force generating capacity. However, ATPase activity of single muscle fibers taken from hibernating bears show a significant reduction in ATPase activity, which is due to a reduced ATP turnover by myosin. By performing a single fiber proteomics analysis, we could determine in a fiber type specific manner that muscle fibers undergo a major remodeling of their proteome. Both type 2A and type 1/2A mixed fibers show a marked reduction in mitochondrial proteins during hibernation, with a decrease in proteins linked to the TCA cycle and mitochondrial translation.
HostingRepository	PRIDE
AnnounceDate	2024-12-06
AnnouncementXML	Submission_2024-12-06_15:43:29.419.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luisa Marie Schmidt
SpeciesList	scientific name: Ursus arctos (Brown bear) (Grizzly bear); NCBI TaxID: 9644;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-26 06:05:36	ID requested
⏵ 1	2024-12-06 15:43:29	announced

Dataset with its publication pending

submitter keyword: bear, hibernation, skinned muscle fibers,single muscle fiber

Bert Blaauw
contact affiliation	Venetian Institute of Molecular Medicine (VIMM), Via Orus 2, 35129, Padova, Italy Department of Biomedical Sciences, 35137, University of Padova, Padova, Italy
contact email	bert.blaauw@unipd.it
lab head
Luisa Marie Schmidt
contact affiliation	CECAD research center, Institue of Genetics, University of Cologne
contact email	luisa.schmidt@uni-koeln.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD050980

PRIDE project URI

• PRIDE
  1. PXD050980
     1. Label: PRIDE project
     2. Name: Resting skeletal muscle reduces ATP turnover during hibernation