PXD050948 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Pulldown of P180/RRBP1 interacting protein |
Description | P180/RRBP1 is an integral ER-resident protein known to be involved in mRNA binding and translation at the ER. We found that P180/RBBP1 is enriched in neuronal axons. To acquire more knowledge about its interactome and confirmation that P180/RRBP1 is interacting with ribosomes, we performed an unbiased screen of P180 interacting proteins by performing streptavidin pulldowns with GFP-biotin (GFPbio/AviTag)-tagged P180 and subsequent mass spectrometry analysis using HEK293T lysates and adult rat brain extracts. Co-transfection with a BirA construct ensures biotinylation ofP180 and allows subsequent streptavidin pulldown. A GFP-biotin/AviTag only construct was used as control conditionfor specificity. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-15 |
AnnouncementXML | Submission_2024-10-15_01:50:11.137.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Max Koppers |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-03-25 03:49:50 | ID requested | |
⏵ 1 | 2024-10-15 01:50:12 | announced | |
Publication List
Koppers M, Ö, zkan N, Nguyen HH, Jurriens D, McCaughey J, Nguyen DTM, Li CH, Stucchi R, Altelaar M, MacGillavry HD, Kapitein LC, Hoogenraad CC, Far, í, as GG, Axonal endoplasmic reticulum tubules control local translation via P180/RRBP1-mediated ribosome interactions. Dev Cell, 59(16):2053-2068.e9(2024) [pubmed] |
10.1016/j.devcel.2024.05.005; |
Keyword List
submitter keyword: brain,P180/RRBP1, HEK293 |
Contact List
Ginny G. Farias |
contact affiliation | Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, The Netherlands |
contact email | g.c.fariasgaldames@uu.nl |
lab head | |
Max Koppers |
contact affiliation | Vrije Universiteit Amsterdam. CNCR, Functional Genomics Department |
contact email | m.koppers@vu.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD050948 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD050948
- Label: PRIDE project
- Name: Pulldown of P180/RRBP1 interacting protein