PXD050946 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Magoh, Y14, eIF4AIII interactomes by TurboID proximity labelling provide no evidence for a canonical EJC in (almost) intron-less trypanosomatids |
Description | In metazoans, mRNA quality is tightly monitored from transcription to translation. A key role lies with the exon junction complex (EJC) that is placed upstream of the exon-exon junction after splicing. The EJC inner core is composed of Magoh, Y14, eIF4AIII and BTZ and the outer core of proteins involved in mRNA splicing (CWC22), export (Yra1), translation (PYM) and non-sense mediated decay (NMD, UPF1/2/3). The protozoan parasite Trypanosoma brucei encodes only two genes with introns, but all mRNAs are processed by trans-splicing. The presence of the three core EJC proteins and a potential BTZ homologue (Rbp25) in trypanosomes has been suggested as an adaptation of the EJC function to mark trans-spliced mRNAs. Here we explore the interactome of Magoh, Y14, eIF4AIII in T. brucei by TurboID proximity labelling. |
HostingRepository | PRIDE |
AnnounceDate | 2024-03-25 |
AnnouncementXML | Submission_2024-03-25_03:14:03.171.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Martin Zoltner |
SpeciesList | scientific name: Trypanosoma brucei; NCBI TaxID: 5691; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-03-25 03:01:26 | ID requested | |
⏵ 1 | 2024-03-25 03:14:03 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: mRNA metabolism, mRNA export control, exon junction complex,Trypanosoma brucei |
Contact List
Dr. Martin Zoltner |
contact affiliation | Head of Drug Discovery and Evaluation Charles University in Prague, BIOCEV Průmyslová 595 252 50 Vestec Czech Republic |
contact email | zoltnerm@natur.cuni.cz |
lab head | |
Martin Zoltner |
contact affiliation | Division of Biological Chemistry & Drug Discovery School of Life Sciences University of Dundee Dundee DD1 5EH |
contact email | m.zoltner@dundee.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD050946
- Label: PRIDE project
- Name: Magoh, Y14, eIF4AIII interactomes by TurboID proximity labelling provide no evidence for a canonical EJC in (almost) intron-less trypanosomatids