PXD050937-1

PXD050937 is an original dataset announced via ProteomeXchange.

Title	Fission yeast Caprin protein is required for efficient heterochromatin establishment.
Description	Heterochromatin is a key feature of eukaryotic genomes that serves important regulatory and structural roles in regions such as centromeres. In fission yeast, maintenance of existing heterochromatic domains relies on positive feedback loops involving histone methylation and non-coding RNAs. However, requirements for de novo establishment of heterochromatin are less well understood. Here, through a cross-based assay we have identified a novel factor influencing the efficiency of heterochromatin establishment. We determine that the previously uncharacterised protein is an ortholog of human Caprin1, an RNA-binding protein linked to stress granule formation. We confirm that the fission yeast ortholog, here named Cpn1, also associates with stress granules, and we uncover evidence of interplay between heterochromatin integrity and ribonucleoprotein (RNP) granule formation, with heterochromatin mutants showing reduced granule formation in the presence of stress, but increased granule formation in the absence of stress. We link this to regulation of non-coding heterochromatic transcripts, since in heterochromatin-deficient cells, absence of Cpn1 leads to hyperaccumulation of centromeric RNAs at centromeres. Together our findings unveil a novel link between RNP homeostasis and heterochromatin assembly, and implicate Cpn1 and associated factors in facilitating heterochromatin establishment by promoting removal of excess transcripts that would otherwise impair assembly processes.
HostingRepository	PRIDE
AnnounceDate	2025-02-14
AnnouncementXML	Submission_2025-02-14_01:30:44.531.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Elizabeth Bayne
SpeciesList	scientific name: Schizosaccharomyces pombe 927; NCBI TaxID: 1264690;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-24 16:51:03	ID requested
⏵ 1	2025-02-14 01:30:44	announced

Dataset with its publication pending

submitter keyword: Caprin, shotgun proteomics,Schizosaccharomyces pombe, RNA, heterochromatin, label-free quantitation

Elizabeth Bayne
contact affiliation	University of Edinburgh
contact email	elizabeth.bayne@ed.ac.uk
lab head
Elizabeth Bayne
contact affiliation	University of Edinburgh
contact email	elizabeth.bayne@ed.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050937
     1. Label: PRIDE project
     2. Name: Fission yeast Caprin protein is required for efficient heterochromatin establishment.