PXD050933 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Phosphoproteome enrichment strategy using complementary chemistries of Fe-NTA and TiO2 followed by LC-MS/MS |
| Description | The global phosphoproteome analysis by liquid chromatography-mass spectrometry (LC-MS) has emerged as an important tool to study cell signalling. However, the coverage of phosphoproteome by LC-MS is limited despite various approaches for phosphoprotein enrichment. Studies in literature have exploited the complementary chemistries of affinity materials like metal (immobilized metal ion affinity chromatography- IMAC) and metal oxides (metal oxide affinity chromatography- MOAC) independently and in a sequential elution approach to improve coverage of phosphoproteins. We demonstrate that further improvement in the enrichment of phosphoproteins can be achieved by using these metals and metal oxides independently and pooling the data. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-05-06 |
| AnnouncementXML | Submission_2025-05-06_13:52:15.606.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Neha Agrawal |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | TripleTOF 5600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-03-24 09:16:01 | ID requested | |
| ⏵ 1 | 2025-05-06 13:52:16 | announced | |
Publication List
| 10.1002/ansa.202400058; |
| Agrawal N, Govekar R, Incremental Modification in the Existing Approaches for Affinity Chromatographic Enrichment of Phosphoproteins Improves Their Profile in Liquid Chromatography-Tandem Mass Spectrometry Analysis. Anal Sci Adv, 6(1):e202400058(2025) [pubmed] |
Keyword List
| submitter keyword: affinity chromatography,Phosphoproteins, enrichment, LC-MS/MS |
Contact List
| Dr. Rukmini Govekar |
|---|
| contact affiliation | Advanced Centre for Treatment, Research and Education in Cancer |
| contact email | rgovekar@actrec.gov.in |
| lab head | |
| Neha Agrawal |
|---|
| contact affiliation | Advanced Centre for Treatment, Research and Education in Cancer |
| contact email | nagrawal9528@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD050933 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD050933
- Label: PRIDE project
- Name: Phosphoproteome enrichment strategy using complementary chemistries of Fe-NTA and TiO2 followed by LC-MS/MS
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