PXD050868-1

PXD050868 is an original dataset announced via ProteomeXchange.

Title	De novo protein synthesis occurs through cytoplasmic translation machinery in mammalian spermatozoa
Description	For decades, it was widely accepted that mammalian spermatozoa were devoid of translational activity due to the functional and morphological changes that occurred during spermatogenesis. However, it was proved that capacitated spermatozoa possess translation activity, which appears to be ensured by mitochondrial ribosomes. Since mitochondrial translation has several particularities, the hypothesis that sperm translation occurs through mitochondrial ribosomes raises some questions. The main objectives of this work were to study the impact of translation inhibition in the bovine spermatozoa proteome and to identify the de novo synthesized proteins by capacitated bovine spermatozoa. To identify which proteins were de novo translated, capacitated bovine spermatozoa were treated with puromycin and puromycin-peptides were immunoprecipitated. To study the impact of translation inhibitors in capacitated sperm proteome, bovine spermatozoa were capacitated in the presence or absence of translation inhibitors - Cycloheximide (CHX, cytoplasmic translation inhibitor) and D-Chloramphenicol (D-CP, mitochondrial translation inhibitor). After capacitation, sperm lysates were analysed by mass spectrometry. We identify nine newly synthesised cytoplasmic proteins by capacitated bovine spermatozoa, which are related to sperm-specific processes. Both translation inhibitors impact differently the sperm proteome leading to the deregulation of cytoplasmic protein related to sperm physiology and function. These results support the existence of cytoplasmic translation in bovine spermatozoa during capacitation.
HostingRepository	PRIDE
AnnounceDate	2025-06-13
AnnouncementXML	Submission_2025-06-13_08:03:57.600.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD050868
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sara Dufour
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-21 10:19:56	ID requested
⏵ 1	2025-06-13 08:03:58	announced

10.6019/PXD050868;

10.1002/jcp.70038;

Corda PO, Silva JV, Almeida CR, Pierre P, Fardilha M, De Novo Protein Synthesis Occurs Through the Cytoplasmic Translation Machinery in Mammalian Spermatozoa. J Cell Physiol, 240(5):e70038(2025) [pubmed]

submitter keyword: spermatozoa

capacitation

protein translation

proteomics

Pedro O. Corda; Margarida Fardilha
contact affiliation	Institute of Biomedicine (iBiMED), Department of Medical Sciences, University of Aveiro (Portugal)
contact email	mfardilha@ua.pt
lab head
Sara Dufour
contact affiliation	VIB-UGent
contact email	sara.dufour@vib-ugent.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050868
     1. Label: PRIDE project
     2. Name: De novo protein synthesis occurs through cytoplasmic translation machinery in mammalian spermatozoa