PXD050843-1

PXD050843 is an original dataset announced via ProteomeXchange.

Title	Long-lived proteins and DNA as candidate predictive biomarkers for tissue associated diseases
Description	Protein turnover, the result of protein synthesis and degradation, is an important mechanism to preserve cellular integrity. The majority of cellular proteins are broken down over a period of hours to weeks to prevent a buildup of proteins that lose their function due to damage caused by misfolding, modifications or degradation. Only a small fraction of the proteome is long-lived. In the current study we have subjected mice to in vivo stable isotope labeling starting at birth until postnatal day 89. Proteins from ten tissues and plasma were then analyzed by shotgun proteomics and interrogated for long-lived proteins (LLPs) by subjecting mass spectra to a database search for non-labeled proteins which yielded 2113 LLPs identifications from 10 mouse tissues. These proteins differed between tissues, with some shared by almost all tissues. Interestingly, a significant percentage of LLPs was detected in plasma. The accumulation of these LLPs may contribute to age-related cardiovascular and other diseases. LLPs identified in the brain were related to neurodegenerative diseases. The relative quantification of non-labeled and labeled DNA-derived deoxynucleosides from the same tissues by mass spectrometry provided information about DNA cellular renewal and showed good correlation with LLPs in the brain. The combined data reveal tissue-specific maps of mouse LLPs that may be involved in pathology due to their low degree of renewal and the resulting increased risk for incurring damage. Tissue-derived peripheral LLPs have potential to serve as biomarkers for aging and age-related disease.
HostingRepository	iProX
AnnounceDate	2024-03-21
AnnouncementXML	Submission_2024-03-20_20:12:03.276.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaosong Liu
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-20 20:04:04	ID requested
⏵ 1	2024-03-20 20:12:03	announced

Dataset with its publication pending

submitter keyword: Long-lived proteins and DNA, tissue, diseases, biomarkers

Yaoyang Zhang
contact affiliation	Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences
contact email	zyy@sioc.ac.cn
lab head
Xiaosong Liu
contact affiliation	Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences
contact email	xiaosong_l@mail.sioc.ac.cn
dataset submitter

iProX dataset URI