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PXD050749-1

PXD050749 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDeep, Unbiased and Quantitative mass spectrometry-based plasma proteome analysis of personalized response to mRNA COVID-19 vaccine
DescriptionGlobal efforts against COVID-19 have vaccinated a significant portion of the world population in recent years. Combating the COVID-19 pandemic with mRNA vaccines playinged a pivotal role in global immunization effort. However, individual responses to these vaccines vary, leading to diverse vaccination efficacy. Despite significant progress, full understanding of the molecular mechanisms driving the personalized immune response to COVID-19 vaccine remains elusive. To address this gap, we combined a novel nanoparticle-based proteomic workflow with tandem mass tag (TMT) labeling, to quantitatively assess the proteomic changes in a cohort of 12 volunteers following two doses of the Pfizer-BioNTech mRNA COVID-19 vaccine. This optimized protocol seamlessly integrates comprehensive proteome analysis with enhanced throughput by leveraging the enrichment of low-abundant plasma proteins by engineered nanoparticles. Our data demonstrate the ability of this nanoparticle-based workflow to quantify over 3,000 proteins from 48 human plasma samples, providing the deepest view into COVID-19 vaccine-related plasma proteome study. We identified 69 proteins exhibiting a boosted response to the vaccine after the second dose. Additionally, 74 proteins were differentially regulated between seven volunteers, who contracted COVID-19 despite receiving two doses of the vaccine, and the ones who did not contract COVID-19. These findings offer valuable insights into individual variability in response to vaccination, demonstrating the potential of personalized medicine approaches in vaccine development.
HostingRepositoryMassIVE
AnnounceDate2025-01-13
AnnouncementXMLSubmission_2025-01-13_08:38:51.403.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAlexandre Rosa Campos
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListOxidation
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-03-18 18:53:41ID requested
12025-01-13 08:38:51announced
Publication List
no publication
Keyword List
submitter keyword: Plasma proteomics, COVID-19 vaccine, Immune response, Nanoparticle-based Workflow, Personalized Response
Contact List
Alex Rosa Campos
contact affiliationSanford Burnham Prebys Medical Discovery Institute
contact emailarosacampos@sbpdiscovery.org
lab head
Alexandre Rosa Campos
contact affiliationSBP Medical Discovery Institute
contact emailarosacampos@sbpdiscovery.org
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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