PXD050749-1
PXD050749 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Deep, Unbiased and Quantitative mass spectrometry-based plasma proteome analysis of personalized response to mRNA COVID-19 vaccine |
Description | Global efforts against COVID-19 have vaccinated a significant portion of the world population in recent years. Combating the COVID-19 pandemic with mRNA vaccines playinged a pivotal role in global immunization effort. However, individual responses to these vaccines vary, leading to diverse vaccination efficacy. Despite significant progress, full understanding of the molecular mechanisms driving the personalized immune response to COVID-19 vaccine remains elusive. To address this gap, we combined a novel nanoparticle-based proteomic workflow with tandem mass tag (TMT) labeling, to quantitatively assess the proteomic changes in a cohort of 12 volunteers following two doses of the Pfizer-BioNTech mRNA COVID-19 vaccine. This optimized protocol seamlessly integrates comprehensive proteome analysis with enhanced throughput by leveraging the enrichment of low-abundant plasma proteins by engineered nanoparticles. Our data demonstrate the ability of this nanoparticle-based workflow to quantify over 3,000 proteins from 48 human plasma samples, providing the deepest view into COVID-19 vaccine-related plasma proteome study. We identified 69 proteins exhibiting a boosted response to the vaccine after the second dose. Additionally, 74 proteins were differentially regulated between seven volunteers, who contracted COVID-19 despite receiving two doses of the vaccine, and the ones who did not contract COVID-19. These findings offer valuable insights into individual variability in response to vaccination, demonstrating the potential of personalized medicine approaches in vaccine development. |
HostingRepository | MassIVE |
AnnounceDate | 2025-01-13 |
AnnouncementXML | Submission_2025-01-13_08:38:51.403.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Alexandre Rosa Campos |
SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
ModificationList | Oxidation |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-03-18 18:53:41 | ID requested | |
⏵ 1 | 2025-01-13 08:38:51 | announced |
Publication List
no publication |
Keyword List
submitter keyword: Plasma proteomics, COVID-19 vaccine, Immune response, Nanoparticle-based Workflow, Personalized Response |
Contact List
Alex Rosa Campos | |
---|---|
contact affiliation | Sanford Burnham Prebys Medical Discovery Institute |
contact email | arosacampos@sbpdiscovery.org |
lab head | |
Alexandre Rosa Campos | |
contact affiliation | SBP Medical Discovery Institute |
contact email | arosacampos@sbpdiscovery.org |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v07/MSV000094352/ |