PXD050745-1

PXD050745 is an original dataset announced via ProteomeXchange.

Title	Establishment of goat mammary organoid cultures modeling the mammary gland development and lactation
Description	The lactating mammary glands in ruminants play a pivotal economic role in agriculture and food nutrition. The need for an improved 3D in vitro model to study its lactating biology highlights the importance of ongoing research in this field. Overcoming these challenges represents a crucial area of research, with the development of protocols for livestock lactating organoids being a significant goal for the future. In this study, we utilized virgin dairy goats as a model and successfully established a mammary organoid system. These organoids, cultured within an extracellular matrix (ECM) gel, maintained a bilayer structure that closely resembled the original mammary tissue architecture. Importantly, the expression patterns of genes related to fatty acid synthesis and milk proteins in lactating organoids closely mirrored those observed in lactating mammary tissues. Proteomic analysis further confirmed that major milk proteins were highly produced in the lactating organoids. In summary, this study successfully developed a mammary organoid culture system that holds significant promise as a novel platform for cell-based milk production.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_13:17:58.359.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hengbo Shi
SpeciesList	scientific name: Capra hircus (Goat); NCBI TaxID: 9925;
ModificationList	No PTMs are included in the dataset
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-18 17:33:28	ID requested
⏵ 1	2025-05-06 13:17:59	announced

Jia L, Zhang W, Luo T, Li Y, Shu J, Strand J, Yue Y, Purup S, Liu J, Shi H, Establishment of goat mammary organoid cultures modeling the mammary gland development and lactation. J Anim Sci Biotechnol, 15(1):124(2024) [pubmed]

10.1186/s40104-024-01084-7;

submitter keyword: DIA, Mammary organoids,Dairy goat

Hengbo Shi
contact affiliation	Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China;
contact email	shihengbo@zju.edu.cn
lab head
Hengbo Shi
contact affiliation	Zhejiang University
contact email	shihengbo@zju.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD050745

PRIDE project URI

• PRIDE
  1. PXD050745
     1. Label: PRIDE project
     2. Name: Establishment of goat mammary organoid cultures modeling the mammary gland development and lactation