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PXD050738-3

PXD050738 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComplexome profiling of nuclear lysates from control and USP39-depleted HeLa cells
DescriptionRNA splicing and protein degradation systems allow the functional adaptation of the proteome in response to changing cellular contexts. However, the regulatory mechanisms connecting these processes remain poorly understood. Here, we show that impaired spliceosome assembly caused by USP39 deficiency leads to a pathogenic splicing profile characterized by the use of cryptic 5′ splice sites. To explore the interactions of USP39 and the effect of its depletion in HeLa cells, we performed complexome profiling of nuclear lysates. We observed most USP39 stably integrated into the tri-snRNP complex and there is almost no free nuclear protein. More importantly, the relative abundance of tri-snRNP spliceosome complex was impaired in USP39-depleted cells. As previous reports indicated, USP39 is a regulator of tri-snRNP stability and its depletion decreased the levels of assembled U4/U6.U5 complexes.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:59:42.796.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD050738
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAlfredo Cabrera-Orefice
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-03-18 16:05:03ID requested
12024-09-25 10:12:44announced
22024-09-27 02:34:26announced2024-09-27: Updated project metadata.
32024-10-22 06:59:43announced2024-10-22: Updated project metadata.
Publication List
10.6019/PXD050738;
Keyword List
submitter keyword: HeLa cells,Complexome profiling, USP39, Nucleus, Spliceosome
Contact List
Alfredo Cabrera-Orefice
contact affiliationFunctional Proteomics Center, Institute for Cardiovascular Physiology, Goethe University, Frankfurt am Main, Germany
contact emailalfredbiomed@gmail.com
lab head
Alfredo Cabrera-Orefice
contact affiliationGoethe University Frankfurt
contact emailalfredbiomed@gmail.com
dataset submitter
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Dataset FTP location
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