PXD050698-1

PXD050698 is an original dataset announced via ProteomeXchange.

Title	Effects of genetic ablation and pharmacological inhibition of HuR on gene expression, iron metabolism and hormone levels.
Description	Background HuR/ELAV1, a ubiquitous RNA-binding protein, belongs to the RNA-binding protein family and is crucial for stabilizing and regulating the translation of various mRNA targets, influencing gene expression. Elevated HuR levels are associated with multiple disorders, including cancer and neurodegenerative diseases. Despite the identification of small molecule inhibitors targeting HuR, their detailed characterization remains limited. Recently, Eltrombopag, an FDA-approved drug for immune thrombocytopenic purpura and chemotherapy-induced thrombocytopenia, emerged as a potential HuR inhibitor. However, the specific molecular pathways influenced by both HuR and Eltrombopag are not fully understood. Results Our study demonstrates that Eltrombopag operates via HuR inhibition, affecting gene expression regulation at the posttranscriptional level. We show that both HuR knockout and Eltrombopag treatment modulate iron metabolism by decreasing ferritin heavy chain (FTH1) and light chain (FTL) synthesis while increasing the expression of Iron-regulatory protein 2 (IRP2), a key regulator of ferritin translation. Additionally, HuR inhibition reduces the levels of Glycoprotein Hormones, Alpha Polypeptide (CGA), a marker associated with hormone-induced tumors, suggesting a potential use of Eltrombopag in treatment of cancers overexpressing CGA. We observed that the main of control is manifested at the level of translation inhibition, with proteosome-mediated regulation also playing an important role. Conclusions These findings uncover novel posttranscriptional mechanisms governed by HuR and its inhibitor, elucidating pathways relevant to HuR-mediated regulation and molecular therapies aimed at targeting this protein.
HostingRepository	PRIDE
AnnounceDate	2024-12-20
AnnouncementXML	Submission_2024-12-20_08:37:59.871.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gracjan Michlewski
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-17 18:45:08	ID requested
⏵ 1	2024-12-20 08:38:00	announced

Dataset with its publication pending

submitter keyword: Iron Metabolism, DIA., ELAVL1, shotgun proteomics,HuR, Eltrombopag

Gracjan Michlewski
contact affiliation	International Institute of Molecular and Cell Biology in Warsaw, Poland
contact email	gmichlewski@iimcb.gov.pl
lab head
Gracjan Michlewski
contact affiliation	International Institute of Molecular and Cell Biology
contact email	gmichlewski@iimcb.gov.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050698
     1. Label: PRIDE project
     2. Name: Effects of genetic ablation and pharmacological inhibition of HuR on gene expression, iron metabolism and hormone levels.