PXD050696-1

PXD050696 is an original dataset announced via ProteomeXchange.

Title	Mouse Exercise & Sedentary Extracellular Vesicles
Description	In our study, we conducted a comprehensive proteomic analysis on small extracellular vesicles (sEVs) and exercise-induced extracellular vesicles (eEVs) to investigate their potential roles and protein cargos that may be pertinent to neurodegenerative diseases, particularly Alzheimer's Disease (AD). We successfully identified 929 proteins within eEVs, with 84 proteins significantly enriched compared to sEVs. These proteins were predominantly associated with established EV markers, validating our EV isolation technique. However, there was limited correlation between these significantly different proteins in eEVs and existing human plasma and EV datasets, except when comparing overall protein profiles with those upregulated in human plasma after exercise, suggesting a unique exercise-induced protein signature in eEVs. Further pathway analysis revealed that proteins differentially expressed between eEVs and sEVs are involved in critical pathways such as mitochondrial biogenesis and integrin signaling. These findings are significant given the central role of dysfunctional mitochondria in AD and the potential of integrin-mediated interactions in directing EV tropism and facilitating neuronal uptake of EVs, which could be crucial for therapeutic interventions. Additionally, our results indicate an enrichment of Mitogen-Activated Protein Kinases (MAPKs) activation in eEVs, aligning with previous studies on the activation of MAPK in muscle regeneration and neuronal healing processes. This suggests that exercise-induced EVs may carry beneficial proteins for neuronal health and recovery, offering new insights into the mechanisms underlying the positive effects of exercise on neurodegenerative diseases. Further investigations are necessary to explore the specific roles of exercise-induced integrins in neuronal uptake and the therapeutic potential of eEVs in AD.
HostingRepository	PRIDE
AnnounceDate	2025-01-06
AnnouncementXML	Submission_2025-01-05_18:42:22.873.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oliver Fuller
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-17 17:37:48	ID requested
⏵ 1	2025-01-05 18:42:23	announced

10.1016/J.ISCI.2025.111752;

submitter keyword: Exactive HF, Exercise, Metabolism,Mouse, Extracellular Vesicles

Mark Anthony Febbraio
contact affiliation	Cellular & Molecular Metabolism Laboratory, Drug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash University.
contact email	mark.febbraio@monash.edu
lab head
Oliver Fuller
contact affiliation	School of Life & Environmental Sciences, Charles Perkins Centre, The University of Sydney
contact email	oliver.fuller@sydney.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD050696

PRIDE project URI

• PRIDE
  1. PXD050696
     1. Label: PRIDE project
     2. Name: Mouse Exercise & Sedentary Extracellular Vesicles