PXD050693 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The lipid droplet protein DHRS3 is a regulator of melanoma cell state |
| Description | Lipid droplets are fat storage organelles composed of a protein envelope which surrounds a lipid rich core. Dynamic regulation of this protein envelope underlies differential lipid droplet formation and function in diverse cell types. In melanoma, the ability to form lipid droplets has been linked to tumor progression and metastasis, but the role of specific lipid droplet proteins is unknown. To address this, we performed the first proteomic analysis of lipid droplets isolated from melanoma cells. We found that several proteins involved in retinoic acid metabolism were associated with the lipid droplet in melanoma. DHRS3, which is involved in the conversion of all-trans-retinal to all-trans-retinol, is significantly upregulated in the MITFLO/undifferentiated melanoma cell state compared to the MITFHI/melanocytic state. Increased DHRS3 expression is sufficient to drive the MITFHI/melanocytic cells to a more undifferentiated and invasive state. These changes are due to retinoic acid mediated regulation of melanocytic genes through the retinoid X receptors (RXR). Our data demonstrate that melanoma cell state can be regulated by expression of lipid droplet proteins which affect downstream retinoid signaling. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-10 |
| AnnouncementXML | Submission_2025-11-09_16:09:16.084.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | henrik molina |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-03-17 11:07:01 | ID requested | |
| ⏵ 1 | 2025-11-09 16:09:17 | announced | |
Publication List
| 10.1111/pcmr.13208; |
| Johns E, Ma Y, Louphrasitthiphol P, Peralta C, Hunter MV, Raymond JH, Molina H, Goding CR, White RM, The Lipid Droplet Protein DHRS3 Is a Regulator of Melanoma Cell State. Pigment Cell Melanoma Res, 38(1):e13208(2025) [pubmed] |
Keyword List
| submitter keyword: lipid droplet, DHRS3 , melanoma, human |
Contact List
| Richard White |
|---|
| contact affiliation | Cancer Biology and Genetics, MSKCC |
| contact email | whiter@mskcc.org |
| lab head | |
| henrik molina |
|---|
| contact affiliation | THE ROCKEFELLER UNIVERSITY |
| contact email | henrik.molina@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD050693
- Label: PRIDE project
- Name: The lipid droplet protein DHRS3 is a regulator of melanoma cell state
