PXD050682-2

PXD050682 is an original dataset announced via ProteomeXchange.

Title	Proteomic characterization of 1,000 human and murine neutrophils freshly isolated from blood and sites of sterile inflammation
Description	Neutrophils, crucial player in inflammation, present a significant challenge in comprehensive molecular characterization due to limited availability (~1,000 cells) in inflamed sites like steady state infiltration. Prior proteomic studies typically required millions of neutrophils, posing difficulties when dealing with limited samples. This study introduces a ‘low-cell proteome’ workflow for neutrophils, integrating S-trap micro column-based digestion with DIA PASEF to investigate their functional dynamics within individual inflamed site.This method enable protein profiling of 1,000 isoated resting circulatory neutrophils with broad dynamic range in a reproducible manner.Furthermore, we applied this integrated approach to investigate the change in neutrophil proteome under different physiological and pathological conditions, including brain infiltration following stroke in mice and transmigration to the oral cavity post-treatment with tabasco in humans. Our comprehensive proteomic analysis provides insights into the distinct behavioral responses of neutrophils in these inflammatory contexts compared to their circulating counterparts.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_07:01:23.987.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Susmita Ghosh
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-16 07:00:38	ID requested
1	2024-10-11 11:51:20	announced
⏵ 2	2024-10-22 07:01:24	announced	2024-10-22: Updated project metadata.

10.1016/J.MCPRO.2024.100858;

submitter keyword: proteomics, inflammation, stroke, low input, DIA PASEF, transmigration,Neutrophil

Albert Sickmann
contact affiliation	Proteomics, Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V., Dortmund, Germany
contact email	sickmann@isas.de
lab head
Susmita Ghosh
contact affiliation	Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V.
contact email	susmita.ghosh@isas.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD050682

PRIDE project URI

• PRIDE
  1. PXD050682
     1. Label: PRIDE project
     2. Name: Proteomic characterization of 1,000 human and murine neutrophils freshly isolated from blood and sites of sterile inflammation