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PXD050625-2

PXD050625 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePairwise screening of viral-bacterial co-infections in vitro reveals molecular interaction points that affect pathogen proliferation and host cell viability
DescriptionThe broadening of accessible methodologies has enabled mechanistic insights into single-pathogen infections, yet the molecular mechanisms underlying co-infections remain largely elusive, despite their clinical frequency and relevance, generally exacerbating symptom severity and fatality. Here, we describe an unbiased in vitro screening of pairwise co-infections in a murine macrophage model, quantifying pathogen proliferation and host cell death in parallel over time. The screen revealed that the majority of interactions are antagonistic for both metrics, highlighting general patterns depending on the pathogen virulence strategy. We subsequently decipher two distinct molecular interaction points: Firstly, murine Adenovirus 3 modifies ASC-dependent inflammasome responses in murine macrophages, altering host cell death and cytokine production, thereby impacting secondary Salmonella infection. Secondly, murine Adenovirus 2 infection triggers upregulation of Mprip, a crucial mediator of phagocytosis, which in turn causes increased Yersinia uptake, specifically in virus pre-infected bone-marrow-derived macrophages. This work therefore encompasses both a first-of-its-kind systematic assessment of host-pathogen-pathogen interactions, and mechanistic insight into molecular mediators during co-infection.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:51:30.358.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPhilipp Walch
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-03-14 09:42:19ID requested
12024-07-25 10:24:30announced
22024-10-22 06:51:30announced2024-10-22: Updated project metadata.
Publication List
10.21203/RS.3.RS-4000173/V1;
Keyword List
submitter keyword: innate immune response,Co-infection, screening, host-pathogen interaction
Contact List
Petr Broz
contact affiliationDepartment of Immunobiology, University of Lausanne, Switzerland
contact emailpetr.broz@unil.ch
lab head
Philipp Walch
contact affiliationUniversity of Lausanne
contact emailphilipp.walch@unil.ch
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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