PXD050612-1

PXD050612 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of mycobacterium marinum culture filtrate
Description	Mycobacterium marinum is a pathogenic bacterium that causes infections in both fish and humans. The PE/PPE protein family is unique to mycobacteria and has been implicated in various aspects of mycobacterial pathogenicity. Understanding the presence and potential function of these proteins in M. marinum is essential for unraveling the mechanisms underlying its virulence. In this study, we conducted a proteomics analysis to investigate the secretion and potential role of the PE/PPE protein family in the culture filtrate of M. marinum. To investigate the presence of PE/PPE proteins in the culture filtrate, we employed a proteomics approach combining Asp-N and trypsin based digestion. Cultures of M. marinum were grown under laboratory conditions, and the culture filtrate was collected and subjected to digestion and LC-MS injection. Our proteomics analysis revealed the secretion of about half PE/PPE proteins in the culture filtrate of M. marinum. Majority of these proteins are confirmed to be involved in EspG5 interaction. The identification of these proteins in the culture filtrate suggests their secretion and potential role in M. marinum. Our proteomics analysis highlights the presence of PE/PPE proteins in the culture filtrate of M. marinum, suggesting their secretion and potential involvement in the pathogenicity of this bacterium.
HostingRepository	PRIDE
AnnounceDate	2025-05-13
AnnouncementXML	Submission_2025-05-13_06:09:14.041.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	lily yan
SpeciesList	scientific name: Mycobacterium marinum (strain ATCC BAA-535 / M); NCBI TaxID: 216594;
ModificationList	carbamoylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-14 02:36:05	ID requested
⏵ 1	2025-05-13 06:09:14	announced

10.3390/ijms25179550;

Yan L, Lai HY, Leung TCN, Cheng HF, Chen X, Tsui SKW, Ngai SM, Au SWN, . Int J Mol Sci, 25(17):(2024) [pubmed]

submitter keyword: Mycobacterium marinum, LC-MS, culture filtrate

Au Wing Ngor Shannon
contact affiliation	Professor, School of Life Sciences, The Chinese University of Hong Kong Director, Centre for Protein Science and Crystallography Assistant Dean (Student Affairs), Faculty of Sciences Fellow, Shaw College
contact email	shannon-au@cuhk.edu.hk
lab head
lily yan
contact affiliation	Department of biochemistry, School of life science, The Chinese University of Hong Kong
contact email	liliyan85@foxmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD050612

PRIDE project URI

• PRIDE
  1. PXD050612
     1. Label: PRIDE project
     2. Name: Proteomics analysis of mycobacterium marinum culture filtrate