PXD050606-1

PXD050606 is an original dataset announced via ProteomeXchange.

Title	The plant-specific protein IQD22 interacts with calmodulins to facilitate hypoxia-induced ethanolic fermentation
Description	Louis Pasteur first reported that living cells switch from aerobic to anaerobic metabolism under low-oxygen conditions. We searched for Arabidopsis thaliana mutants with downregulated expression of hypoxia-induced ALCOHOL DEHYDROGENASE 1 (ADH1), encoding a key enzyme in ethanolic fermentation. This screen identified mutants in IQ DOMAIN containing protein 22 (IQD22). The iqd22 mutants were hypersensitive to submergence and hypoxic stress, whereas IQD22 overexpressors were more tolerant of both compared to wild type. Moreover, under hypoxia, IQD22 interacted with calmodulins (CaMs) in vivo and facilitated their association with ADH1, stimulating its activity. Metabolic profiling revealed that hypoxia caused significant increases of glycolytic metabolites, but significantly lower ethanol in iqd22-2 mutant compared to the wild type. Furthermore, deleting ADH1 suppressed the improved hypoxia-tolerance phenotype of IQD22 overexpressors. Our findings thus shed light on the IQD22–CaM–ADH1 regulatory module that mediates calcium-dependent activation of anaerobic respiration to control metabolic flux during hypoxia.
HostingRepository	iProX
AnnounceDate	2024-03-13
AnnouncementXML	Submission_2025-01-02_19:31:03.328.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shanshan Zhao
SpeciesList	scientific name: Arabidopsis thaliana; NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-13 19:01:54	ID requested
⏵ 1	2025-01-02 19:31:03	announced

Dataset with its publication pending

submitter keyword: ADH1, calmodulins, ethanolic fermentation, hypoxia, IQD22, metabolic shift

Shi Xiao
contact affiliation	Sun Yat-sen University
contact email	xiaoshi3@mail.sysu.edu.cn
lab head
Shanshan Zhao
contact affiliation	Sun Yat-sen University
contact email	zhaoshsh6@mail2.sysu.edu.cn
dataset submitter

iProX dataset URI