PXD050587-1

PXD050587 is an original dataset announced via ProteomeXchange.

Title	Quantitative assessment of microbiomes using ultra-fast MS/MS-free metaproteomics: model 1 and 3
Description	This study presents an adaptation of the novel ultrafast MS1-based method called DirectMS1 to characterization of the single microorganisms and microbiomes. Using DirectMS1 we demonstrate the blind identification (with no prior knowledge of the bacterias in the sample) of a single bacterial species and microbial compositions using two-step database search against the microbial part of SwissProt and TrEMBL (62Gb database size), the evaluation of fold changes in biomass of microorganisms between the metaproteomic samples, and the assessment of metabolic activity of Rhodococcus species responding to a change in growth conditions and cold shock.
HostingRepository	PRIDE
AnnounceDate	2026-08-07
AnnouncementXML	Submission_2026-08-07_02:14:30.536.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tomiris Kusainova
SpeciesList	scientific name: Priestia aryabhattai; NCBI TaxID: NEWT:412384; scientific name: Gordonia alkanivorans; NCBI TaxID: NEWT:84096; scientific name: Gordonia amicalis; NCBI TaxID: NEWT:89053; scientific name: Rhodococcus erythropolis; NCBI TaxID: NEWT:1833; scientific name: Rhodococcus opacus; NCBI TaxID: NEWT:37919;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-13 07:16:41	ID requested
⏵ 1	2026-08-07 02:14:31	announced
2	2026-08-07 03:12:52	announced	2026-08-07: Updated project metadata.

Dataset with its publication pending

submitter keyword: metaproteomics, MS1-only, microbiomes, DirectMS1

Dr Irina A. Tarasova
contact affiliation	INEPCP FRCCP RAS, Moscow, Russia
contact email	iatarasova@yandex.ru
lab head
Tomiris Kusainova
contact affiliation	V. L. Talrose Institute for Energy Problems of Chemical Physics, N. N. Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, 119334 Moscow, Russia Moscow Institute of Physics and Technology, 141701 Dolgoprudny, Russia
contact email	kusainova7531@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD050587

PRIDE project URI

• PRIDE
  1. PXD050587
     1. Label: PRIDE project
     2. Name: Quantitative assessment of microbiomes using ultra-fast MS/MS-free metaproteomics: model 1 and 3