PXD050585-1

PXD050585 is an original dataset announced via ProteomeXchange.

Title	Identification of components in protein complex of influenza A virus PA protein
Description	Influenza A virus is mainly transmitted through the respiratory route and can cause severe illness in humans. Proteins encoded by influenza A virus can interact with cellular factors and dysregulate host biological processes to facilitate viral replication and pathogenicity. The influenza viral PA protein is not only a subunit of influenza viral polymerase but also a virulence factor involved in pathogenicity during infection. To explore the role of the influenza virus PA protein in regulating host biological processes, we conducted immunoprecipitation and LC-MS/MS to globally identify cellular factors that interact with the PA proteins of the influenza A H1N1, 2009 pandemic H1N1, H3N2, and H7N9 viruses. The results demonstrated that proteins located in the mitochondrion, proteasome, and nucleus are associated with the PA protein. We further discovered that the PA protein is located in mitochondria by immunofluorescence and mitochondrial fractionation and that overexpression of the PA protein reduces mitochondrial respiration. In addition, our results revealed the interaction between PA and the mitochondrial matrix protein PYCR2 and the antiviral role of PYCR2 during influenza A virus replication. Moreover, we found that the PA protein could also trigger autophagy and disrupt mitochondrial homeostasis. Overall, our research revealed the impacts of the influenza A virus PA protein on mitochondrial function and autophagy.
HostingRepository	PRIDE
AnnounceDate	2024-06-23
AnnouncementXML	Submission_2024-06-23_01:43:14.827.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chih-Ching Wu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-13 05:44:33	ID requested
⏵ 1	2024-06-23 01:43:15	announced

Wu CC, Tam EH, Shih YY, Lin YR, Hsueh PC, Shen HY, Woung CH, Wang LT, Tsai JC, Lin SJ, Chang CR, Ke PY, Kuo RL, Exploration of influenza A virus PA protein-associated cellular proteins discloses its impact on mitochondrial function. Virus Res, 345():199387(2024) [pubmed]

10.1016/j.virusres.2024.199387;

ProteomeXchange project tag: Human Proteome Project

submitter keyword: spectral counting-based quantification, polymerase acidic protein (PA),interactome, influenza A virus

Chih-Ching Wu
contact affiliation	Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Taiwan
contact email	luckywu@mail.cgu.edu.tw
lab head
Chih-Ching Wu
contact affiliation	Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University
contact email	luckywu@mail.cgu.edu.tw
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050585
     1. Label: PRIDE project
     2. Name: Identification of components in protein complex of influenza A virus PA protein