PXD050581-1

PXD050581 is an original dataset announced via ProteomeXchange.

Title	Protease Regulation by Subtrate O-Glycosylation
Description	In this study, we systematically assessed the fine-tuning of limited proteolysis in the extracellular space by glycosylation, one of the most widely observed PTMs, using cells, and applying the Terminal Amine Labeling of Substrates (TAILS) approach, a quantitative proteomics workflow for the system-wide assessment of protein N termini and protease cleavage events in complex biological samples. In addtion, we complement the study with DIA analysis, PRM and glycoproteomics analysis for further validation of the initial observations.
HostingRepository	PRIDE
AnnounceDate	2024-08-11
AnnouncementXML	Submission_2024-08-10_16:05:37.080.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Elizabeta Madzharova
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-13 03:43:18	ID requested
⏵ 1	2024-08-10 16:05:37	announced
2	2024-10-22 06:54:36	announced	2024-10-22: Updated project metadata.

10.1002/pro.5128;

Madzharova E, Sabino F, Kalogeropoulos K, Francavilla C, Auf dem Keller U, Substrate O-glycosylation actively regulates extracellular proteolysis. Protein Sci, 33(8):e5128(2024) [pubmed]

submitter keyword: PTMs,proteomics, degradomics

Chiara Francavilla
contact affiliation	Department of Biotechnology and Biomedicine, Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark
contact email	chiafra@dtu.dk
lab head
Elizabeta Madzharova
contact affiliation	Technical University of Denmark
contact email	elimad@dtu.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD050581

PRIDE project URI

• PRIDE
  1. PXD050581
     1. Label: PRIDE project
     2. Name: Protease Regulation by Subtrate O-Glycosylation