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PXD050557-1

PXD050557 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAutoregulation of tubulin mRNA via reversible TTC5 sequestration by soluble αβ-tubulins
DescriptionThe microtubule cytoskeleton, built from heterodimers of α and β-tubulins, is critically important to physically organize cells, mediate intracellular transport and power cell division. The availability of soluble αβ-tubulins influences the biomechanical properties and dynamic functions of microtubules. When present in excess, soluble αβ-tubulins induce degradation of their encoding mRNAs. This process involves the specificity factor TTC5, which recognizes nascent tubulins and recruits effectors that degrade the mRNA. But how TTC5 activity is regulated is unknown. Our biochemical and structural proteomic approaches reveal that soluble αβ-tubulins sequester TTC5 at steady-state, repressing its activity. The carboxy-terminal domain of TTC5 acts as a molecular switch, toggling between soluble αβ-tubulin-bound and nascent tubulin-bound states. Loss-of-function mutantions that abolish sequestration by soluble αβ-tubulins constitutively activate TTC5, leading to diminished tubulin mRNA levels and compromised microtubule-dependent chromosome segregation during cell division. Our findings provide a paradigm for how cells regulate the activity of a specificity factor to adapt posttranscriptional regulation of gene expression to cellular needs.
HostingRepositoryPRIDE
AnnounceDate2024-10-18
AnnouncementXMLSubmission_2024-10-18_05:34:46.375.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterCatarina Franco
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-03-12 08:00:01ID requested
12024-10-18 05:34:46announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: αβ-tubulins, TTC5
Contact List
Ramanujan Hegde
contact affiliationMedical Research Council Laboratory of Molecular Biology, Cambridge CB2 0QH, United Kingdom
contact emailrhegde@mrc-lmb.cam.ac.uk
lab head
Catarina Franco
contact affiliationMRC Laboratory of Molecular Biology
contact emailcffranco@mrc-lmb.cam.ac.uk
dataset submitter
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Dataset FTP location
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