PXD050530-3

PXD050530 is an original dataset announced via ProteomeXchange.

Title	Ion Mobility-Tandem Mass Spectrometry of Mucin-type O-Glycans
Description	The dense O-glycosylation of mucins plays an important role in the defensive properties of the mucus hydrogel. Aberrant glycosylation is often correlated with inflammation and pathology such as COPD, cancer, and Crohn’s disease. The inherent complexity of glycans and the diversity in the O-core structure constitute fundamental challenges for the analysis of mucin-type O-glycans. Due to coexistence of multiple isomers, multidimensional workflows such as LC-MS are required. To separate the highly polar carbohydrates, porous graphitized carbon is often used as a stationary phase. However, LC-MS workflows are time-consuming and lack reproducibility. Here we present a rapid alternative for separating and identifying O-glycans released from mucins based on trapped ion mobility mass spectrometry. Compared to established LC-MS, the acquisition time is reduced from an hour to two minutes. To test the validity, the developed workflow was applied to sputum samples from cystic fibrosis patients to map O-glycosylation features associated with disease.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:34:02.757.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marieluise Kirchner
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; complex glycosylation; deamidated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-11 06:17:18	ID requested
1	2024-03-19 04:36:47	announced
2	2024-03-28 09:56:49	announced	2024-03-28: Updated project metadata.
⏵ 3	2024-10-22 06:34:03	announced	2024-10-22: Updated project metadata.

10.1038/s41467-024-46825-4;

Bechtella L, Chunsheng J, Fentker K, Ert, ü, rk GR, Safferthal M, Polewski Ł, G, ö, tze M, Graeber SY, Vos GM, Struwe WB, Mall MA, Mertins P, Karlsson NG, Pagel K, Ion mobility-tandem mass spectrometry of mucin-type O-glycans. Nat Commun, 15(1):2611(2024) [pubmed]

submitter keyword: O-glycans

ion mobility

mass spectrometry

isomeric separation

DDA

Proteome

Kevin Pagel (Glycomics), Philipp Mertins (Proteomics)
contact affiliation	Kevin Pagel: Institute of Chemistry and Biochemistry, Freie Universität Berlin, Altensteinstraße 23A, 14195 Berlin, Germany. Fritz Haber Institute of the Max Planck Society, Faradayweg 4‑6, 14195 Berlin, Germany. Philipp Mertins (Philipp.mertins@mdc-berlin.de) : Max Delbrueck Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany. Berlin Institute of Health (BIH), Berlin, Germany.
contact email	kevin.pagel@fu-berlin.de
lab head
Marieluise Kirchner
contact affiliation	Proteomics Platform, BIH@Charite
contact email	marieluise.kirchner@mdc-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD050530

PRIDE project URI

• PRIDE
  1. PXD050530
     1. Label: PRIDE project
     2. Name: Ion Mobility-Tandem Mass Spectrometry of Mucin-type O-Glycans