PXD050513-2

PXD050513 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry of HCT116 cells
Description	HCT116 cells were transfected with flag-pcDNA3.1 plasmids or flag-MST3 plasmids. Whole-cell extracts were prepared with a lysis buffer, lysed on ice for 10 min, centrifuged at 12,000 rpm for 10 min at 4°C, and the supernatant was collected. The anti-Flag antibody and protein A/G beads were added and incubated for 6-8 h and washed five times with wash buffer. Proteins were eluted with 0.15 M glycine (pH 2.5-3.1), and the supernatant was collected and immediately added to 1/10 volume of neutralization buffer (0.1 M NaOH) to adjust the pH of the eluted product to neutral. Mass spectrometry analysis was performed.
HostingRepository	iProX
AnnounceDate	2024-03-09
AnnouncementXML	Submission_2025-08-28_23:58:58.987.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shiyang Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-10 20:34:36	ID requested
1	2024-11-05 18:55:19	announced
⏵ 2	2025-08-28 23:58:59	announced	2025-08-29: add info.

Wang S, Wu X, Bi W, Xu J, Hou L, Li G, Pan Y, Zhang H, Li M, Du S, Zhang M, Liu D, Jin S, Shi X, Tian Y, Shuai J, Plikus MV, Song M, Zhou Z, Yu L, Lv C, Yu Z, ROS-induced cytosolic release of mitochondrial PGAM5 promotes colorectal cancer progression by interacting with MST3. Nat Commun, 16(1):1406(2025) [pubmed]

submitter keyword: Mass spectrometry data

Zhengquan Yu
contact affiliation	Center for Life Science
contact email	zyu@cau.edu.cn
lab head
Shiyang Wang
contact affiliation	Center for Life Science
contact email	yy520521@cau.edu.cn
dataset submitter

iProX dataset URI