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PXD050490-1

PXD050490 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFunctional proteomics-based investigation of the cellular response to farnesyl transferase inhibition in lung cancer (subcellular localization and relocalization analysis)
DescriptionThe subcellular localization of proteins can be highly dynamic and regulated by post-translational modifications. Farnesylation is a lipid modification that enables anchoring of proteins to cellular membranes, and thus, spatially resolved signaling and activity. Due to the importance of farnesylation for cellular functions, farnesyltransferase inhibitors (FTIs) including tipifarnib were developed and are being tested in oncology clinical trials. The current lack of knowledge on the functional impact of FTIs on hundreds of farnesylation substrates limits response prediction and their use in cancer precision medicine. Here, we report a global functional proteomics investigation of farnesylation and the impact of tipifarnib in multiple lung cancer cell lines. This dataset includes global mapping of protein localization using our SubCellBarCode method, and analyses of tipifarnib-dependent protein relocalization.
HostingRepositoryPRIDE
AnnounceDate2025-05-19
AnnouncementXMLSubmission_2025-05-19_05:33:41.163.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGeorgios Mermelekas
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-03-08 12:55:27ID requested
12025-05-19 05:33:42announced
Publication List
10.1016/j.isci.2025.111864;
Pan Y, Berkovska O, Marathe S, Mermelekas G, Gudoityte G, Wolide AD, Arslan T, Seashore-Ludlow B, Lehti, รถ J, Orre LM, Functional-proteomics-based investigation of the cellular response to farnesyltransferase inhibition in lung cancer. iScience, 28(2):111864(2025) [pubmed]
Keyword List
submitter keyword: proteomics, tipifarnib,Lung cancer, human, farnesylation, subcellular, HiRIEF, cell line, LC-MS/MS
Contact List
Lukas Orre
contact affiliationDepartment of oncology and pathology, Karolinska Institutet, Stockholm, Sweden
contact emailLukas.orre@ki.se
lab head
Georgios Mermelekas
contact affiliationKarolinska Institutet
contact emailgeorgios.mermelekas@scilifelab.se
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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