PXD050483 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Autoregulation of tubulin mRNA via reversible TTC5 sequestration by soluble ab-tubulins |
Description | Microtubule cytoskeleton, built from heterodimers of α and β-tubulins, is critically important to physically organize cells, mediate intracellular transport and power cell division. The availability of soluble αβ-tubulins influences the biomechanical properties and functions of microtubules. When present in excess, soluble αβ-tubulins induce degradation of their encoding mRNAs. This process involves the activation of a specificity factor TTC5, which recognizes nascent tubulins and recruits effectors that degrade the mRNA. But how TTC5 activity is regulated is unknown. Our biochemical and structural proteomic approaches reveal that soluble αβ-tubulins at steady-state levels sequester TTC5, repressing its activity. The carboxy-terminal domain of TTC5 acts as a molecular switch, toggling between soluble αβ-tubulin-bound and nascent tubulin-bound states. Loss of sequestration by soluble αβ-tubulins constitutively activates TTC5, leading to diminished tubulin mRNA levels and compromised microtubule-dependent chromosome segregation during cell division. Our findings provide a paradigm for how cells regulate the activity of a specificity factor to adapt posttranscriptional regulation of gene expression to cellular needs. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-18 |
AnnouncementXML | Submission_2024-10-18_07:35:54.399.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD050483 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Oscar Vadas |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-03-08 06:52:49 | ID requested | |
⏵ 1 | 2024-10-18 07:35:54 | announced | |
Publication List
Keyword List
submitter keyword: Microtubules, TTC5, HDX-MS, mRNA, Tubulin |
Contact List
Oscar Vadas |
contact affiliation | University of Geneva, Faculty of Medicine, Protein platform |
contact email | oscar.vadas@unige.ch |
lab head | |
Oscar Vadas |
contact affiliation | University of Geneva, Faculty of Medicine |
contact email | oscar.vadas@unige.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD050483
- Label: PRIDE project
- Name: Autoregulation of tubulin mRNA via reversible TTC5 sequestration by soluble ab-tubulins