PXD050458-1

PXD050458 is an original dataset announced via ProteomeXchange.

Title	Unveiling the Proteome of the Fasting Heart: Insights into HIF-1 Pathway Regulation.
Description	Fasting is a common dietary intervention known for its protective effects against metabolic and cardiovascular diseases. While its effects are mostly systemic, understanding tissue-specific changes in the heart is crucial for the identification of the mechanisms underlying fasting-induced cardioprotection. In this study, we performed a proteomic analysis to elucidate the proteome of the fasting heart. Our investigation identified a total of 4,652 proteins, with 127 exhibiting down-regulation and 118 showing up-regulation after fasting. Annotation analysis highlighted significant changes in processes such as lipid metabolism, the peroxisome pathway, and reactive oxygen species metabolism. Notably, the HIF-1 signaling pathway emerged as one of the focal points, with five affected proteins identified. Further experiments demonstrated down-regulation of HIF-1α at both transcript and protein levels. Intriguingly, while gene expression of Egln3 decreased, its protein product PHD3 remained unaffected by fasting. These findings underscore the regulation of the well-established cardioprotective HIF-1 signaling within the heart during 3-day fasting.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_14:37:18.497.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marek Vrbacky
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	methylthiolated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-07 13:39:10	ID requested
⏵ 1	2024-10-08 14:37:18	announced
2	2024-10-22 07:00:56	announced	2024-10-22: Updated project metadata.

10.3389/FPHYS.2024.1462014;

submitter keyword: fasting, HIF-1, proteome,heart, PHD3

Marketa Hlavackova
contact affiliation	Institute of Physiology of the Czech Academy of Sciences Laboratory of Developmental Cardiology
contact email	Marketa.Hlavackova@fgu.cas.cz
lab head
Marek Vrbacky
contact affiliation	Czech Academy of Sciences
contact email	proteom.krc@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050458
     1. Label: PRIDE project
     2. Name: Unveiling the Proteome of the Fasting Heart: Insights into HIF-1 Pathway Regulation.