PXD050456-2

PXD050456 is an original dataset announced via ProteomeXchange.

Title	SYNTAXIN OF PLANTS 132 underpins secretion of cargoes associated with salicylic acid signaling and pathogen defense
Description	In Arabidopsis, SYNTAXIN OF PLANTS 132 (SYP132) is an evolutionarily ancient SNARE that functions with syntaxins SYP121 and SYP122 at the plasma membrane. Whereas SYP121 and SYP122 mediate overlapping secretory pathways, albeit with differences in their importance in plant-environment interactions, the SNARE SYP132 is absolutely essential for plant development and survival. SYP132 promotes endocytic traffic of the plasma membrane H+-ATPase AHA1 and aquaporin PIP2;1, and it coordinates plant growth and bacterial pathogen immunity through PATHOGENESIS-RELATED1 (PR1) secretion. Yet, little else is known about SYP132 cargoes. Here, we used advanced quantitative Tandem Mass Tagging (TMT) mass spectrometry (MS) combined with immunoblot assays to track native secreted cargo proteins in the leaf apoplast from wild type, syp121/syp122 double mutant and Arabidopsis transgenics that exhibit altered SYP132 functions, SYP132-OX and SYP132Deterrent.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_07:01:17.403.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rucha Karnik
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-07 11:35:20	ID requested
1	2024-10-11 05:08:09	announced
⏵ 2	2024-10-22 07:01:17	announced	2024-10-22: Updated project metadata.

10.1093/PLPHYS/KIAE541;

submitter keyword: secretion, growth, pathogen defence, salicylic acid,SNARE

Dr Rucha
contact affiliation	Plant Science Group, School of Molecular Biosciences, College of Medical, Veterinary and Life Sciences, Bower Building, University of Glasgow, University Avenue, G12 8QQ Glasgow, UK
contact email	rucha.karnik@glasgow.ac.uk
lab head
Rucha Karnik
contact affiliation	University of Glasgow
contact email	rucha.karnik@glasgow.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050456
     1. Label: PRIDE project
     2. Name: SYNTAXIN OF PLANTS 132 underpins secretion of cargoes associated with salicylic acid signaling and pathogen defense