PXD050456 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | SYNTAXIN OF PLANTS 132 underpins secretion of cargoes associated with salicylic acid signaling and pathogen defense |
Description | In Arabidopsis, SYNTAXIN OF PLANTS 132 (SYP132) is an evolutionarily ancient SNARE that functions with syntaxins SYP121 and SYP122 at the plasma membrane. Whereas SYP121 and SYP122 mediate overlapping secretory pathways, albeit with differences in their importance in plant-environment interactions, the SNARE SYP132 is absolutely essential for plant development and survival. SYP132 promotes endocytic traffic of the plasma membrane H+-ATPase AHA1 and aquaporin PIP2;1, and it coordinates plant growth and bacterial pathogen immunity through PATHOGENESIS-RELATED1 (PR1) secretion. Yet, little else is known about SYP132 cargoes. Here, we used advanced quantitative Tandem Mass Tagging (TMT) mass spectrometry (MS) combined with immunoblot assays to track native secreted cargo proteins in the leaf apoplast from wild type, syp121/syp122 double mutant and Arabidopsis transgenics that exhibit altered SYP132 functions, SYP132-OX and SYP132Deterrent. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_07:01:17.403.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Rucha Karnik |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion; LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-03-07 11:35:20 | ID requested | |
1 | 2024-10-11 05:08:09 | announced | |
⏵ 2 | 2024-10-22 07:01:17 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: secretion, growth, pathogen defence, salicylic acid,SNARE |
Contact List
Dr Rucha |
contact affiliation | Plant Science Group, School of Molecular Biosciences, College of Medical, Veterinary and Life Sciences, Bower Building, University of Glasgow, University Avenue, G12 8QQ Glasgow, UK |
contact email | rucha.karnik@glasgow.ac.uk |
lab head | |
Rucha Karnik |
contact affiliation | University of Glasgow |
contact email | rucha.karnik@glasgow.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD050456
- Label: PRIDE project
- Name: SYNTAXIN OF PLANTS 132 underpins secretion of cargoes associated with salicylic acid signaling and pathogen defense