PXD050442-1

PXD050442 is an original dataset announced via ProteomeXchange.

Title	Cytoplasmic DIS3 degrades circular RNAs independently of the exosome
Description	Circular RNAs (circRNAs) are frequently generated during splicing. Their physiological roles, however, remain largely elusive. Some specific circRNAs function as microRNA (miRNA) or RNA binding protein (RBP) sponges or encode for proteins and are actively translated. Due to the lack of accessible ends, circRNAs are resistant to exonucleolytic cleavage and thus are more stable than linear RNAs. Nevertheless, circRNAs are most likely turned over by cellular degradation pathways. Here, we used a biochemical purification strategy to find nucleases that could degrade circRNAs. We identified the exosome-associated nuclease DIS3, which contains an endonucleolytic PIN domain and an exonuclease domain. We show that DIS3 can degrade circRNAs independently of the exosome in vitro. Although moderately, RNA-seq reveals that selected circRNAs are stabilized when DIS3 is reduced in vivo. Based on our data, we propose a model, in which cytoplasmic DIS3 contributes to the turnover of circRNAs in human cells.
HostingRepository	PRIDE
AnnounceDate	2025-05-05
AnnouncementXML	Submission_2025-05-05_04:00:04.309.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD050442
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Astrid Bruckmann
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidoethyl-L-cysteine; deamidated residue; iodoacetamide derivatized residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-07 06:21:47	ID requested
⏵ 1	2025-05-05 04:00:04	announced

10.6019/PXD050442;

submitter keyword: exosome,circular RNAs, DIS3, degradation, circRNA

Astrid Bruckmann
contact affiliation	University Regensburg, Biochemistry I,Mass spectrometry group
contact email	astrid.bruckmann@ur.de
lab head
Astrid Bruckmann
contact affiliation	University Regensburg
contact email	astrid.bruckmann@ur.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050442
     1. Label: PRIDE project
     2. Name: Cytoplasmic DIS3 degrades circular RNAs independently of the exosome