PXD050438-1

PXD050438 is an original dataset announced via ProteomeXchange.

Title	Global quantitative MS proteome analysis of in vitro activated murine primary B cells of Dis3G766R/+ and Dis3+/+ genotypes
Description	DIS3 is a ribonucleolytic subunit of the exosome complex responsible for RNA degradation in the nucleus, predominantly affecting steady state levels of non-coding transcripts. The effect of the mild mutant Dis3 G766R allele, clinically related t multiple myeloma, in a heterozygous state on protein-coding transcripts is negligible. In this experiment, we assayed the proteome of in vitro activated primary B cells 72 hours after their activation with LPS (20 µg/ml) and IL-4 (20 ng/ml). TMT-based global quantification of protein levels was performed in Dis3G766R/+ and Dis3+/+ samples in triplicates per condition. This dataset is directly related to a transcriptomic analysis available under a GEO accession number GSE155631 containing RNA-seq and DRIP-seq data of corresponding samples
HostingRepository	PRIDE
AnnounceDate	2026-02-13
AnnouncementXML	Submission_2026-02-13_04:53:45.422.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrzej Dziembowski
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-07 04:52:57	ID requested
⏵ 1	2026-02-13 04:53:45	announced

Dataset with its publication pending

submitter keyword: mouse, Dis3G766R/+ and Dis3+/+ genotypes, LPS and IL-4 activation, B cells

Andrzej Dziembowski
contact affiliation	International Institute of Molecular and Cell Biology in Warsaw, Poland
contact email	adziembowski@iimcb.gov.pl
lab head
Andrzej Dziembowski
contact affiliation	International Institute of Molecular and Cell Biology in Warsaw, Poland
contact email	adziembowski@iimcb.gov.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050438
     1. Label: PRIDE project
     2. Name: Global quantitative MS proteome analysis of in vitro activated murine primary B cells of Dis3G766R/+ and Dis3+/+ genotypes