PXD050418-1

PXD050418 is an original dataset announced via ProteomeXchange.

Title	Proteomic Insights into Psychrophile Growth in Perchlorate-Amended Subzero Conditions: Implications for Martian Life Detection
Description	Forty-five Colwellia psychrerythraea 34H (Cp34H) samples were individually prepared for mass spectrometry proteomics to identify proteomic effects of perchlorate oxy-ions and putative perchlorate-tolerant biosignatures. Cultures of Cp34H were grown in half-strength 2216 medium at -1°C to late log phase (to an optical density (OD) at 600 nm of 0.2-0.3, three proteomic samples are from this initial culture. 100 µl of this culture was used to inoculate 1 ml of 1) half-strength 2216 medium (control) and 2) half-strength 2216 medium amended with perchlorate ions (WCL medium), at a 30:1 ratio (2216:WCL) and incubated at -1℃ and -5℃ for 15 days. Twenty-one proteomic samples are control samples, grown in half strength 2216 medium, and the other twenty-one proteomic samples were grown in half strength 2216 medium with perchlorate ions (WCL) at a 30:1 ratio (2216:WCL). Biological triplicate samples from certain time points (7,9, and 11 at -1℃; 5,9,11,13 at -5℃) were selective for proteomic analysis.
HostingRepository	PRIDE
AnnounceDate	2025-02-14
AnnouncementXML	Submission_2025-02-14_11:43:53.782.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anais Gentilhomme
SpeciesList	scientific name: Colwellia psychrerythraea 34H; NCBI TaxID: 167879;
ModificationList	acetylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-06 14:10:20	ID requested
⏵ 1	2025-02-14 11:43:54	announced

Dataset with its publication pending

submitter keyword: Perchlorate, Colwellia psychrerythraea 34H

Brook Nunn
contact affiliation	University of Washington- Department of Genome Sciences
contact email	brookh@uw.edu
lab head
Anais Gentilhomme
contact affiliation	University of Washington
contact email	gentia@uw.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050418
     1. Label: PRIDE project
     2. Name: Proteomic Insights into Psychrophile Growth in Perchlorate-Amended Subzero Conditions: Implications for Martian Life Detection