PXD050410-1

PXD050410 is an original dataset announced via ProteomeXchange.

Title	Comparing the proteome of human induced pluripotent stem cells cultured during normoxic versus hypoxic conditions
Description	Human induced pluripotent stem cells (hiPSCs) have become an invaluable tool to study molecular disease mechanisms on a human genetic background as they can be differentiated in different cell types including cardiac myocytes. One major downside that has decelerated the research performed in hiPSCs is that during propagation of these cells, changes in the karyotype of these cells have been observed. Interestingly, when hiPSCs are being cultured under hypoxic conditions and not as commonly done under normoxic conditions, hiPSCs grow faster to confluency and also changes in the karyotype of these cells are less frequently observed.
HostingRepository	PRIDE
AnnounceDate	2025-01-07
AnnouncementXML	Submission_2025-01-07_05:41:16.291.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ilka Wittig
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-06 10:43:17	ID requested
⏵ 1	2025-01-07 05:41:17	announced

10.1016/j.redox.2024.103352;

Raabe J, Wittig I, Laurette P, Stathopoulou K, Brand T, Schulze T, Klampe B, Orthey E, Cabrera-Orefice A, Meisterknecht J, Thiemann E, Laufer SD, Shibamiya A, Reinsch M, Fuchs S, Kaiser J, Yang J, Zehr S, Wrona KM, Lorenz K, Lukowski R, Hansen A, Gilsbach R, Brandes RP, Ulmer BM, Eschenhagen T, Cuello F, Physioxia rewires mitochondrial complex composition to protect stem cell viability. Redox Biol, 77():103352(2024) [pubmed]

submitter keyword: normoxia,Proteome remodeling, hypoxia, stem cells

Friederike Cuello
contact affiliation	Institute of Experimental Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
contact email	f.cuello@uke.de
lab head
Ilka Wittig
contact affiliation	Functional Proteomics, Goethe University, Frankfurt am Main , Germany
contact email	wittig@med.uni-frankfurt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD050410

PRIDE project URI

• PRIDE
  1. PXD050410
     1. Label: PRIDE project
     2. Name: Comparing the proteome of human induced pluripotent stem cells cultured during normoxic versus hypoxic conditions