PXD050396 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Phosphoproteomic analysis reveals the diversity of signaling behind ErbB inhibitor-induced phenotypes |
| Description | Effects of kinase inhibitors on the phosphoproteome have been rarely investigated at a whole organism level. Here we performed a phosphoproteomic analysis in embryonic zebrafish to identify the signaling pathways perturbed by the ErbB receptor tyrosine kinase inhibitors at the whole organism level. The phosphorylation of the proteins associated with the PI3K/Akt, p38 MAPK, Notch, Hippo/Yap and β-catenin signaling pathways were differentially regulated by the ErbB inhibitors. Gene set enrichment analyses indicated differential neurological and myocardial phenotypes of different ErbB inhibitors. To assess the neurological and myocardial effects, motility and ventricle growth assays were performed on zebrafish embryos treated with the ErbB and downstream signaling pathway inhibitors. The treatment with the inhibitors targeting the PI3K/Akt, p38 MAPK and Notch signaling pathways along with AG1478 and Lapatinib perturbed the motility and ventricle wall growth of zebrafish embryos. Taken together, these results indicate that inhibitors with the same primary targets can affect different signaling pathways while eliciting similar physiological phenotypes. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-12-29 |
| AnnouncementXML | Submission_2025-12-28_17:58:10.750.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Johannes Merilahti |
| SpeciesList | scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: NEWT:7955; |
| ModificationList | phosphorylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-03-06 02:41:40 | ID requested | |
| ⏵ 1 | 2025-12-28 17:58:11 | announced | |
Publication List
| Vaparanta K, Song Z, Farahani I, Jokilammi A, Merilahti J, Ö, rling J, Virtanen N, Haapaniemi P, Sahlgren C, Elenius K, Paatero I, Phosphoproteomic analysis reveals the diversity of signaling behind ErbB-inhibitor-induced phenotypes. FEBS J, 292(24):6557-6575(2025) [pubmed] |
| 10.1111/febs.70197; |
Keyword List
| submitter keyword: ErbB inhibitor, signaling pathways, zebrafish embryos,Phosphoproteome |
Contact List
| Ilkka Paatero |
|---|
| contact affiliation | Turku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, Finland. |
| contact email | ilkka.paatero@utu.fi |
| lab head | |
| Johannes Merilahti |
|---|
| contact affiliation | Turku Bioscience
University of Turku |
| contact email | johannes.merilahti@utu.fi |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD050396
- Label: PRIDE project
- Name: Phosphoproteomic analysis reveals the diversity of signaling behind ErbB inhibitor-induced phenotypes
