PXD050384-1

PXD050384 is an original dataset announced via ProteomeXchange.

Title	SARS-CoV-2 Spike Protein in the Skull-Meninges-Brain Axis
Description	COVID-19 has been associated with a variety of neurological symptoms, including brain fog and brain tissue loss. This raises concerns about the virus's acute and potential chronic impacts on the brain. Using whole mouse and human tissue clearing, we determined all potential tissues targeted by the SARS-CoV-2 and showed that the spike protein accumulates in the skull-meninges-brain axis in both mice and humans. Injection of the spike protein into the mouse skull alone was sufficient to cause cell death in the brain. Notably, we found spike protein in the skull of ~60% of deceased people who had COVID-19 in the past. Mass spectrometry-based proteomics in mice and humans revealed dysregulated inflammatory and vascular pathways throughout tissues in the skull-meninges-brain axis. Overall, SARS-CoV-2 spike protein accumulation in the CNS borders might be involved in the immediate and long-term consequences in the brain parenchyma, and this may present diagnostic and therapeutic opportunities.
HostingRepository	PRIDE
AnnounceDate	2024-12-03
AnnouncementXML	Submission_2024-12-02_18:07:16.889.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ali Erturk
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF SCP

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-05 18:19:19	ID requested
⏵ 1	2024-12-02 18:07:17	announced

Rong Z, Mai H, Ebert G, Kapoor S, Puelles VG, Czogalla J, Hu S, Su J, Prtvar D, Singh I, Sch, ä, dler J, Delbridge C, Steinke H, Frenzel H, Schmidt K, Braun C, Bruch G, Ruf V, Ali M, S, ü, hs KW, Nemati M, Hopfner F, Ulukaya S, Jeridi D, Mistretta D, Caliskan Ö S, Wettengel JM, Cherif F, Kolabas ZI, Molbay M, Horvath I, Zhao S, Krahmer N, Yildirim A Ö, Ussar S, Herms J, Huber TB, Tahirovic S, Schwarzmaier SM, Plesnila N, H, ö, glinger G, Ondruschka B, Bechmann I, Protzer U, Elsner M, Bhatia HS, Hellal F, Ert, ü, rk A, Persistence of spike protein at the skull-meninges-brain axis may contribute to the neurological sequelae of COVID-19. Cell Host Microbe, 32(12):2112-2130.e10(2024) [pubmed]

10.1016/j.chom.2024.11.007;

submitter keyword: COVID-19, Skull, DIA, Meninges, Brain, SARS-CoV-2, LC-MS/MS

Ali Erturk
contact affiliation	Institute for tissue engineering and regenerative medicine, Helmholtz Center Munich
contact email	ali.erturk@helmholtz-munich.de
lab head
Ali Erturk
contact affiliation	Institute for Tissue Engineering and Regenerative Medicine, Helmholtz Munich
contact email	ali.erturk@helmholtz-munich.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD050384

PRIDE project URI

• PRIDE
  1. PXD050384
     1. Label: PRIDE project
     2. Name: SARS-CoV-2 Spike Protein in the Skull-Meninges-Brain Axis