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PXD050380-1

PXD050380 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBerry e-cigarette vapour impairs alveolar macrophage mobility and infection clearance
DescriptionIn our lower respiratory tract, the alveolar spaces are divided from the bloodstream and the external environment by only a few microns of interstitial tissue. Alveolar macrophages (AMs) defend this delicate mucosal surface from invading infections by regularly patrolling the site. AMs have three behaviour modalities to achieve this goal: extending cell protrusions to sample surrounding areas, squeezing the whole cell body between alveoli, and patrolling by moving the cell body around each alveolus. In this study we found Rho GTPase, cell division control protein 42 (CDC42) expression significantly decreased after berry-flavoured e-cigarette (e-cig) exposure. This resulted in a shift in AM behaviour from squeezing to probing. Changes in AM behaviour led to a reduction in the clearance of inhaled bacteria, Pseudomonas aeruginosa. These findings shed light on pathways involved in AM migration and highlight the harmful impact of e-cigarette vaping on alveolar macrophage function.
HostingRepositoryPRIDE
AnnounceDate2024-09-04
AnnouncementXMLSubmission_2024-09-04_15:05:57.066.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLuiz Gustavo de Almeida
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-03-05 14:39:35ID requested
12024-09-04 15:05:57announced
Publication List
10.1073/PNAS.2406294121;
Keyword List
submitter keyword: e-cigarette exposures,alveolar macrophages, infection control
Contact List
Antoine Dufour
contact affiliationDepartment of Biochemistry and Molecular Biology, University of Calgary, Alberta, Canada; Department of Physiology and Pharmacology, University of Calgary, Alberta, Canada; The Hotchkiss Brain Institute, University of Calgary, Alberta, Canada; McCaig Institute for Bone and Joint Health, University of Calgary, Alberta, Canada
contact emailantoine.dufour@ucalgary.ca
lab head
Luiz Gustavo de Almeida
contact affiliationUniversity of Calgary
contact emailluizgustavo.biotec@hotmail.com
dataset submitter
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Dataset FTP location
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