PXD050367-1

PXD050367 is an original dataset announced via ProteomeXchange.

Title	Complete list of canonical post-transcriptional modifications in Bacillus subtilis ribosome.
Description	Using bottom-up oligonucleotide LC-MS/MS in combination with stable isotope labeling, chemical derivatization, and bioinformatics variable modification search, a total of 25 modifications were mapped to the sequences of 16S and 23S RNA in B. subtilis. 10 modification sites, previously identified by others using alternative methods were independently confirmed using targeted MS/MS data analysis. Modification types, namely base/ribose methylation, pseudouridines and dihydrouridine, and modification positions are fully consistent with prior experimental evidence and agree well with modifications that were annotated in E. coli and other bacteria species.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:20:30.816.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anna Popova
SpeciesList	scientific name: Bacillus subtilis PY79; NCBI TaxID: 1415167; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationList	methylated residue; dimethylated residue
Instrument	impact II; 6520A Quadrupole Time-of-Flight LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-05 07:19:51	ID requested
⏵ 1	2024-10-08 11:20:31	announced

10.1093/NAR/GKAE626;

submitter keyword: pseudouridine, base and ribose methylations, RNA modifications,ribosomal RNA

James Williamson
contact affiliation	Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
contact email	jrwill@scripps.edu
lab head
Anna Popova
contact affiliation	Scripps Research
contact email	popova@scripps.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD050367

PRIDE project URI

• PRIDE
  1. PXD050367
     1. Label: PRIDE project
     2. Name: Complete list of canonical post-transcriptional modifications in Bacillus subtilis ribosome.