PXD050362 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Improved Mass Spectrometry-based methods to study histone propionylation |
Description | Histone post-translational modifications (PTMs) have crucial roles in a multitude of cellular processes, their aberrant levels have been linked with numerous diseases, including cancer. Although histone PTM investigations have focused so far on methylations and acetylations, alternative long-chain acylations emerged as new dimension, as they are linked to cellular metabolic states and affect gene expression through mechanisms distinct from those regulated by acetylation. Mass spectrometry (MS) is the most powerful, comprehensive and unbiased method to study histone PTMs. Typical protocols for histone PTM analysis do not allow identification of naturally occurring propionylation and butyrylation. Here, we present improved state-of-the-art sample preparation and analysis protocols to quantitate these classes of modifications. After testing different derivatization methods coupled to protease digestion, we profiled common histone PTMs and histone acylations in seven mouse tissues and human normal and tumor breast clinical samples, obtaining a map of propionylations and butyrylations found in different tissue contexts. A quantitative histone PTM analysis also revealed a contribution of histone acylations in discriminating different tissues and breast cancer samples from the normal counterpart. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:49:26.204.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Roberta Noberini |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monomethylated residue; acylated residue; acetylated residue; N6,N6,N6-trimethyl-L-lysine; N6,N6-dimethyl-L-lysine |
Instrument | Q Exactive HF; Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-03-05 06:36:44 | ID requested | |
1 | 2024-10-07 13:52:21 | announced | |
⏵ 2 | 2024-10-22 06:49:27 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: histone post-translational modifications, propionylation, acylation |
Contact List
Tiziana Bonaldi |
contact affiliation | European Instutute of Oncology, Milan |
contact email | tiziana.bonaldi@ieo.it |
lab head | |
Roberta Noberini |
contact affiliation | Istituto Europeo di Oncologia |
contact email | roberta.noberini@ieo.it |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD050362
- Label: PRIDE project
- Name: Improved Mass Spectrometry-based methods to study histone propionylation