PXD050279-2

PXD050279 is an original dataset announced via ProteomeXchange.

Title	BAG3 regulates cilia homeostasis of glioblastoma via its WW domain
Description	The multidomain protein BAG3 exerts pleiotropic oncogenic functions in many tumor entities including glioblastoma (GBM). Here we compared BAG3 protein-protein interactions in either adherently cultured or stem-like cultured U251 GBM cells. In line with BAG3´s putative role in stemness, identified interactors in sphere-cultured cells included different stem cell markers (SOX2, OLIG2, NES), while interactomes of adherent BAG3-proficient cells indicated a shift towards involvement of BAG3 in regulation of cilium assembly (ACTR3, ARL3). Applying a set of BAG3 deletion constructs we could demonstrate that none of the domains except the WW domain are required for suppression of cilia formation by full-length BAG3 in U251 and U343 cells. In line with the established regulation of the Hippo pathway by this domain, we could show that the WW mutant fails to rescue YAP1 nuclear translocation. BAG3 depletion reduced activation of a YAP1/AURKA signaling pathway and induction of PLK1. Collectively, our findings point to a complex interaction network of BAG3 with several pathways regulating cilia homeostasis, involving processes related to ciliogenesis and cilium degradation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:35:36.201.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thorsten Mosler
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-02 02:35:01	ID requested
1	2024-04-09 07:01:56	announced
⏵ 2	2024-10-22 06:35:36	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: cilia homeostasis, stemness, YAP1, WW domain,Glioblastoma, BAG3, YAP1 signaling, primary cilia

Donat Kögel
contact affiliation	Department of Neurosurgery, Experimental Neurosurgery, University Hospital, Goethe University, Frankfurt am Main, Germany German Cancer Consortium (DKTK), Partner Site Frankfurt, Frankfurt am Main, Germany German Cancer Research Center DKFZ, Heidelberg, Germany
contact email	koegel@em.uni-frankfurt.de
lab head
Thorsten Mosler
contact affiliation	Goethe University Clinics Frankfurt
contact email	thorstenmosler@googlemail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050279
     1. Label: PRIDE project
     2. Name: BAG3 regulates cilia homeostasis of glioblastoma via its WW domain