PXD050261-1

PXD050261 is an original dataset announced via ProteomeXchange.

Title	Profiling Cullin4-E3 ligases interactomes and their rewiring in influenza A virus infection
Description	Understanding the integrated regulation of cellular processes during viral infection is crucial for developing host-targeted approaches. We have previously reported that an optimal in-vitro infection by influenza A (IAV) requires three components of Cullin 4-RING E3 ubiquitin ligases (CRL4), namely the DDB1 adaptor and two Substrate Recognition Factors (SRF), DCAF11 and DCAF12L1, which mediate non-degradative poly-ubiquitination of the PB2 subunit of the viral polymerase. However, the impact of IAV infection on the CRL4 interactome remains elusive. Here, using Affinity Purification coupled with Mass Spectrometry (AP-MS) approaches, we identified cellular proteins interacting with these CRL4 components in IAV-infected and non-infected contexts. IAV infection induces significant modulations in protein interactions, resulting in a global loss of DDB1 and DCAF11 interactions, and an increase of DCAF12L1-associated proteins. The distinct rewiring of CRL4’s associations upon infection impacted cellular proteins involved in protein folding, ubiquitination, translation, splicing, and stress responses. Using a split-nanoluciferase-based assay, we identified direct partners of CRL4 components, representing potential substrates or regulators of CRL4 complexes. Our findings unravel the dynamic remodeling of the proteomic landscape of CRL4’s E3 ubiquitin ligases during IAV infection, likely involved in shaping a cellular environment conducive to viral replication and offers potential for the exploration of future host-targeted antiviral therapeutic strategies.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_13:28:32.120.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thibaut Douché
SpeciesList	scientific name: Influenza A virus (A/WSN/1933(H1N1)); NCBI TaxID: 382835; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitination signature dipeptidyl lysine; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-01 02:01:30	ID requested
⏵ 1	2025-05-06 13:28:33	announced

Dugied G, Douche T, Dos Santos M, Giai Gianetto Q Q, Cassonnet C, Vuillier F, Cassonnet P, Jacob Y, van der Werf S, Komarova A, Matondo M, Karim M, Demeret C, Profiling Cullin4-E3 Ligases Interactomes and Their Rewiring in Influenza A Virus Infection. Mol Cell Proteomics, 23(11):100856(2024) [pubmed]

10.1016/j.mcpro.2024.100856;

submitter keyword: Human, AP-MS, Q Exactive Plus, influenza virus

Caroline Demeret
contact affiliation	Interactomics, RNA and Immunity Laboratory, Institut Pasteur, 75015 Paris, France
contact email	caroline.demeret@pasteur.fr
lab head
Thibaut Douché
contact affiliation	Institut Pasteur
contact email	thibaut.douche@pasteur.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050261
     1. Label: PRIDE project
     2. Name: Profiling Cullin4-E3 ligases interactomes and their rewiring in influenza A virus infection