PXD050241-1

PXD050241 is an original dataset announced via ProteomeXchange.

Title	One-Pot Time-Induced Proteome Integral Solubility Alteration (OPTI-PISA) assay for automated and sensitive drug target identification
Description	Proteome Integral Solubility Alteration (PISA) assay is widely used for identifying drug targets, but it is labour-, sample and time-consuming. Aiming at enabling automation and greatly reducing the sample amount, we developed One-Pot Time-Induced (OPTI)-PISA. Here we demonstrate OPTI-PISA performance on identifying targets of multiple drugs in cell lysate and scaling down the sample amount to sub-microgram levels, making PISA method suitable to NanoProteomics.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_13:45:45.004.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhaowei Meng
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-29 05:13:28	ID requested
⏵ 1	2025-05-06 13:45:45	announced

10.1021/acs.analchem.4c05127;

Meng Z, Saei AA, Lyu H, Gaetani M, Zubarev RA, One-Pot Time-Induced Proteome Integral Solubility Alteration Assay for Automated and Sensitive Drug-Target Identification. Anal Chem, 96(48):18917-18921(2024) [pubmed]

submitter keyword: drug target, Proteome Integral Solubility Alteration (PISA),One Pot, automation

Roman A. Zubarev
contact affiliation	Division of Chemistry I, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
contact email	roman.zubarev@ki.se
lab head
Zhaowei Meng
contact affiliation	Department of Medical Biophysics and Biochemistry, Karolinska Institutet
contact email	zhaowei.meng@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD050241

PRIDE project URI

• PRIDE
  1. PXD050241
     1. Label: PRIDE project
     2. Name: One-Pot Time-Induced Proteome Integral Solubility Alteration (OPTI-PISA) assay for automated and sensitive drug target identification