PXD050178-1
PXD050178 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A prokaryotic E2 enzyme imitates the ubiquitin pathway to regulate the antiviral cGAS |
| Description | The cyclic oligonucleotide-based anti-phage signalling system (CBASS) is a typeof innate prokaryotic immune system. Composed of a cyclic GMP-AMP synthase (cGAS) and CBASS-associated proteins, CBASS utilizes cyclic oligonucleotides to activate antiviral immunity. One major class of CBASS contains a homolog of eukaryotic ubiquitin-conjugating enzymes, which is either an E1-E2 fusion or a single E2. However, the functions of single E2s in CBASS remain elusive. Here, we report that a bacterial E2 enzyme regulates cGAS by imitating the ubiquitination cascade. This includes the processing of the cGAS C-terminus, conjugation of cGAS to a cysteine residue, ligation of cGAS to a lysine residue, cleavage of the isopeptide bond, and poly-cGASylation. The poly-cGASylation activates cGAS to produce cGAMP, which acts as an antiviral signal and leads to cell death. Thus, our findings reveal a unique regulatory role of E2 in CBASS. |
| HostingRepository | iProX |
| AnnounceDate | 2024-02-27 |
| AnnouncementXML | Submission_2024-02-26_23:27:42.414.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Xian Wei |
| SpeciesList | scientific name: Serratia marcescens LCT-SM213; NCBI TaxID: 1162294; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | LTQ Orbitrap Velos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-02-26 23:27:23 | ID requested | |
| ⏵ 1 | 2024-02-26 23:27:42 | announced | |
| 2 | 2024-05-09 20:55:05 | announced | 2024-05-10: Update information. |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: cGAS, cGASylation, CABSS |
Contact List
| Xiaoyun Liu | |
|---|---|
| contact affiliation | Peking University Health Science Center |
| contact email | xiaoyun.liu@bjmu.edu.cn |
| lab head | |
| Xian Wei | |
| contact affiliation | Peking University Health Science Center |
| contact email | xianwei@pku.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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