PXD050174-1

PXD050174 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	mRNA-LNP HIV-1 trimer boosters elicit precursors to bnAbs
Description	Germline-targeting (GT) HIV vaccine strategies are predicated on deriving broadly neutralizing antibodies (bnAbs) through multiple boost immunogens. However, as the recruitment of memory B cells (MBCs) to germinal centers (GCs) is inefficient and may be derailed by serum antibody-induced epitope masking, driving further B cell receptor (BCR) modification in GC-experienced B cells after boosting poses a challenge. Using humanized Ig knockin mice, we found that GT protein trimer immunogen N332-GT5 could prime inferred-germline precursors to the V3-glycan-targeted bnAb BG18, and that B cells primed by N332-GT5 were effectively boosted by either of two novel protein immunogens designed to have minimum cross-reactivity with the off target V1-binding responses. The delivery of the prime and boost immunogens as mRNA-LNPs generated long-lasting GCs, somatic hypermutation, and affinity maturation, and may, therefore, be an effective tool in HIV vaccine development.
HostingRepository	MassIVE
AnnounceDate	2024-05-03
AnnouncementXML	Submission_2024-05-03_10:47:37.009.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sabyasachi Baboo
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Human immunodeficiency virus; NCBI TaxID: 12721;
ModificationList	Oxidation; 2-pyrrolidone-5-carboxylic acid (Gln); HexNAc; 1x(18)O labeled deamidated L-asparagine
Instrument	Q Exactive HF-X

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-26 19:58:55	ID requested
⏵ 1	2024-05-03 10:47:37	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: DeGlyPHER, N-glycans, HIV Env Trimer, mRNA vaccine, V3-glycan epitope, bNAb, germline targeting

submitter keyword: DeGlyPHER, N-glycans, HIV Env Trimer, mRNA vaccine, V3-glycan epitope, bNAb, germline targeting

Contact List

John R. Yates III
contact affiliation	The Scripps Research Institute
contact email	jyates@scripps.edu
lab head
Sabyasachi Baboo
contact affiliation	The Scripps Research Institute, La Jolla, CA
contact email	sbaboo@scripps.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v07/MSV000094176/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v07/MSV000094176/