PXD050155 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Drug-induced oxidative stress actively prevents caspase activation and hepatocyte apoptosis |
Description | Cell death is a fundamental process for health and disease. Emerging research unveiled numerous distinct cell death modalities with similar and intertwined signaling pathways, but different outcomes, rising the need to understand the decision points during cell death signaling. Paracetamol (Acetaminophen, APAP)-induced hepatocyte death includes several apoptotic processes, but eventually ends as oncotic necrosis without any caspase activation. Here, we studied this paradoxical form of cell death and revealed that APAP not only fails to activate caspases, but also strongly impedes their activation upon classical apoptosis induction. We explored different hypotheses and deciphered that high oxidative stress consistently blocks caspase activity. Importantly, caspase inhibition and the associated switch from apoptotic to necrotic cell death is reversible through the administration of antioxidants. Thus, exemplified by APAP-induced cell death, our study stresses that the cellular redox status is a critical decision maker between different forms of cell death as it directly affects caspase activity. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:59:33.961.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Florian Stengel |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | dihydroxylated residue; S-nitrosyl-L-cysteine; monohydroxylated residue; L-cysteic acid (L-cysteine sulfonic acid); L-cysteine glutathione disulfide |
Instrument | Q Exactive HF; Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-02-26 09:04:21 | ID requested | |
1 | 2024-09-24 05:41:17 | announced | |
⏵ 2 | 2024-10-22 06:59:34 | announced | 2024-10-22: Updated project metadata. |
Publication List
Lambrecht R, Jansen J, Rudolf F, El-Mesery M, Caporali S, Amelio I, Stengel F, Brunner T, Drug-induced oxidative stress actively prevents caspase activation and hepatocyte apoptosis. Cell Death Dis, 15(9):659(2024) [pubmed] |
10.1038/s41419-024-06998-8; |
Keyword List
submitter keyword: glutathione,Paracetamol/APAP, necrosis, oxidative stress, apoptosis, caspases |
Contact List
Prof. Dr. Florian Stengel |
contact affiliation | Department of Biology, Universitätsstraße 10, 78457 Konstanz, Germany |
contact email | florian.stengel@uni.kn |
lab head | |
Florian Stengel |
contact affiliation | Universität Konstanz |
contact email | florian.stengel@uni-konstanz.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD050155
- Label: PRIDE project
- Name: Drug-induced oxidative stress actively prevents caspase activation and hepatocyte apoptosis