PXD050106-2

PXD050106 is an original dataset announced via ProteomeXchange.

Title	Unraveling soybean responses to early and late Tetranychus urticae (Acari: Tetranychidae) infestation
Description	Soybean is one of the most important sources of food, protein, and oil in the world. Reductions in grain number and quality are caused by different biotic stresses. One of the most common is the phytophagous mite Tetranychus urticae Koch (Acari: Tetranychidae), which inhibits plant growth and grain production. The identification of plant responses to early and late T. urticae infestation is important for a better understanding of the mite-plant interaction. We therefore aimed to evaluate the physiological and molecular responses of soybean plants to mite infestation for 5 and 21 days. Visual and microscopic symptoms of leaf damage, H2O2 accumulation, and lipid peroxidation increased consistently throughout the infestation period, while shoot length/dry weight, chlorophyll level, and number of days to reach specific developmental stages were negatively affected by T. urticae infestation. Using proteomic analysis, we identified 185 and 266 differentially abundant proteins after early (5 days) and late (21 days) mite infestation, respectively, which suggests a complex remodeling of diverse metabolic pathways. GO, KEGG, and protein-protein interaction analyses indicated that photorespiration, chlorophyll synthesis, amino acid metabolism, Krebs cycle/energy production, mitochondrial translation, nucleotide salvage, PS II assembly, and reductive pentose-P cycle are all impacted after both early and late infestation. Specific metabolic pathways modified only after early infestation include cell wall modification, cytoskeleton composition, cell division, and lysine/histidine metabolism, while JA biosynthesis, antioxidant system, S-adenosyl methionine cycle, PS II repair, cysteine/methionine/glutathione/ascorbate/-linolenic acid/selenocompound metabolism, arginine biosynthesis, and proteasome are modified only after late infestation. These differentially abundant proteins can be used as biotechnological tools in future breeding programs aiming at increased resistance to mite infestation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:58:35.698.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Raul Sperotto
SpeciesList	scientific name: Glycine max; NCBI TaxID: 3847;
ModificationList	No PTMs are included in the dataset
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-24 10:32:55	ID requested
1	2024-09-16 10:40:35	announced
⏵ 2	2024-10-22 06:58:36	announced	2024-10-22: Updated project metadata.

10.1111/PLB.13717;

submitter keyword: defense

mite infestation

proteomic analysis

soybean

Tetranychus urticae.

Raul Antonio Sperotto
contact affiliation	Universidade Federal de Pelotas (UFPel), Programa de Pós-Graduação em Fisiologia Vegetal (PPGFV), Prédio 14, Sala 305, Bairro Campus Universitário, Capão do Leão, RS - CEP: 96.160-000
contact email	rasperotto@univates.br
lab head
Raul Sperotto
contact affiliation	Federal University of Pelotas
contact email	rasperotto@univates.br
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD050106

PRIDE project URI

• PRIDE
  1. PXD050106
     1. Label: PRIDE project
     2. Name: Unraveling soybean responses to early and late Tetranychus urticae (Acari: Tetranychidae) infestation