PXD050098-1

PXD050098 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Hydrophilic interaction chromatography coupled to ultraviolet photodissociation affords identification, localization, and relative quantitation of glycans on intact glycoproteins
Description	Protein glycosylation is implicated in a wide array of diseases, yet glycoprotein analysis remains elusive owing to the extreme heterogeneity of glycans including microheterogeneity at the same amino acid residue (glycosite). Top-down mass spectrometry (MS) allows precise identification and localization of glycans on intact proteins, and coupling top-down MS with chromatography allows time-resolved characterization of glycoforms. Here, we couple ultraviolet photo-dissociation (UVPD) to hydrophilic interaction chromatography (HILIC) to advance the characterization of glycoproteins ranging from 15-34 kDa, offering site localization of glycans, providing sequence coverages up to 93% and relative quan-titation of individual glycoforms.
HostingRepository	jPOST
AnnounceDate	2024-02-24
AnnouncementXML	Submission_2024-02-23_21:10:18.720.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Virginia James
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxymethyl-L-cysteine; 2-pyrrolidone-5-carboxylic acid (Gln)
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-23 19:56:22	ID requested
⏵ 1	2024-02-23 21:10:19	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Protein glycosylation, top-down mass spectrometry, ultraviolet photodissociation

submitter keyword: Protein glycosylation, top-down mass spectrometry, ultraviolet photodissociation

Contact List

Jennifer Brodbelt
lab head
Virginia James
contact affiliation	UT-Austin
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002959/