PXD050096 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Ogasawara et al_photo-stereoprobes |
Description | Chemical proteomics enables the global assessment of small molecule-protein interactions in native biological systems and has emerged as a versatile approach for ligand discovery. The range of small molecules explored by chemical proteomics has, however, been limited. Here, we describe a diversity-oriented synthesis (DOS)-inspired library of stereochemically-defined compounds bearing diazirine and alkyne units for UV light-induced covalent modification and click chemistry enrichment of interacting proteins, respectively. We find that these ‘photo-stereoprobes’ interact in a stereoselective manner with hundreds of proteins from various structural and functional classes in human cells and demonstrate that these interactions can form the basis for high-throughput screening-compatible nanoBRET assays. Integrated phenotypic analysis and chemical proteomics identified photo-stereoprobes that modulate autophagy by engaging the mitochondrial serine protease CLPP. Our findings show the utility of photo-stereoprobes for expanding the ligandable proteome, furnishing target engagement assays, and discovering and characterizing bioactive small molecules by cell-based screening. |
HostingRepository | PRIDE |
AnnounceDate | 2024-05-31 |
AnnouncementXML | Submission_2024-05-31_10:14:18.006.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Daisuke Ogasawara |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-02-23 14:29:36 | ID requested | |
⏵ 1 | 2024-05-31 10:14:18 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: proteomics, diazirine, chemical biology, photo-reactive probe, phenotypic screening |
Contact List
Benjamin F. Cravatt |
contact affiliation | Department of Chemistry, Scripps Research |
contact email | cravatt@scripps.edu |
lab head | |
Daisuke Ogasawara |
contact affiliation | The Scripps Research Institute |
contact email | daisuke@scripps.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD050096
- Label: PRIDE project
- Name: Ogasawara et al_photo-stereoprobes