PXD050095-1

PXD050095 is an original dataset announced via ProteomeXchange.

Title	Functional Effects of Extracellular Vesicles Altered by a Per- and Polyfluoroalkyl Substance Mixture: Liver Cell Proteomic Expression Alterations
Description	Extracellular vesicles (EVs) have emerged as key molecules in cellular processes and diseases. The intersection of environmental exposures and EVs has recently gained attention, with EVs being highlighted as biomarkers of exposure, as well as mediators of environmental exposure-induced disease. The present study aims to evaluate whether EVs released from HepG2 liver cells can affect the biology of separate recipient HepG2 cells, measured through changes in global cellular protein expression. The hypothesis specifically evaluated in this study was that EVs released from PFAS-mixture treated liver cells are biologically active and will alter protein expression in separate liver cells related to chronic liver diseases and cancer. To evaluate this hypothesis, HepG2 cells representing “parent cells” were exposed to an equimolar PFAS mixture containing perfluorooctanesulfonic acid (PFOS), perfluorooctanoic acid (PFOA), and perfluorohexanoic acid (PFHxA). EVs were isolated from parent cells and used to treat separate HepG2 liver cells, representing “target cells”, to evaluate changes in resulting proteomic expression profiles. Results from this demonstrated that EVs released from PFAS-treated HepG2 cells cause unique protein expression changes in separate HepG2 cells including numerous proteins associated with hepatic cancer, non-alcoholic fatty liver disease, and other hepatic diseases. These findings provide important context to previously observed changes in EV release and molecular content, highlighting the role EVs in a novel mechanism of PFAS-induced liver toxicity.
HostingRepository	PRIDE
AnnounceDate	2025-11-20
AnnouncementXML	Submission_2025-11-20_12:41:14.603.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Angie Mordant
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-23 13:00:49	ID requested
⏵ 1	2025-11-20 12:41:15	announced

Dataset with its publication pending

submitter keyword: in vitro, Environmental Health, Extracellular Vesicles, Proteomics,PFAS, Liver

Julia Rager
contact affiliation	University of North Carolina at Chapel Hill
contact email	jrager@unc.edu
lab head
Angie Mordant
contact affiliation	UNC Proteomics Core, Department of Pharmacology, University of North Carolina at Chapel Hill
contact email	angie_mordant@med.unc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD050095

PRIDE project URI

• PRIDE
  1. PXD050095
     1. Label: PRIDE project
     2. Name: Functional Effects of Extracellular Vesicles Altered by a Per- and Polyfluoroalkyl Substance Mixture: Liver Cell Proteomic Expression Alterations