PXD050087-2

PXD050087 is an original dataset announced via ProteomeXchange.

Title	Purification of time-resolved insulin granules reveals proteomic and lipidomic changes during granule aging
Description	Endocrine cells employ regulated exocytosis of secretory granules to secrete hormones and neurotransmitters. Secretory granule exocytosis depends on spatio-temporal variables such as proximity to the plasma membrane and age, with newly-generated granules being preferentially released. Despite recent advances, we lack a comprehensive view about the molecular composition of insulin granules and associated changes over their lifetime. Here we report a strategy for the purification of insulin secretory granules of distinct age from insulinoma INS-1 cells. Tagging the granule-resident protein phogrin with a cleavable CLIP tag, we obtain intact fractions of age-distinct granules for proteomic and lipidomic analyses. We find that the lipid composition changes
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:36:04.360.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mario Oroshi
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	time-of-flight

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-23 07:43:41	ID requested
1	2024-04-15 07:10:32	announced
⏵ 2	2024-10-22 06:36:04	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: ARHG, proteomics, lipidomics, CLIP tag,Insulin granule, KIF5b, RAB3a, organelle purification

Matthias Mann
contact affiliation	Director Dept. Proteomics and Signal Transduction Max Planck Institute of Biochemistry
contact email	mmann@biochem.mpg.de
lab head
Mario Oroshi
contact affiliation	Proteomics
contact email	oroshi@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD050087

PRIDE project URI

• PRIDE
  1. PXD050087
     1. Label: PRIDE project
     2. Name: Purification of time-resolved insulin granules reveals proteomic and lipidomic changes during granule aging