PXD050085-1

PXD050085 is an original dataset announced via ProteomeXchange.

Title	An interphase actin wave promotes mitochondrial content mixing and 2 organelle homeostasis
Description	Across the cell cycle, mitochondrial dynamics are regulated by a cycling wave of actin polymerization/depolymerization. In metaphase, this wave induces the assembly of actin comet tails on mitochondria that propel these organelles to drive spatial mixing,resulting in their equitable inheritance by daughter cells. In contrast, during interphasethe cycling actin wave promotes localized mitochondrial fission. Here, we identify the F-actin nucleator/elongator FMNL1 as a positive regulator of the wave. Depletion of FMNL1 ablates the actin wave, allowing us to assess the functional consequences in interphase cells. FMNL1-depleted cells exhibit decreased mitochondrial polarization, decreased mitochondrial oxygen consumption, and increased production of reactive oxygen species. Accompanying these changes is a loss of hetero-fusion of wave-fragmented mitochondria. Thus we propose that the interphase actin wave maintains mitochondrial homeostasis by promoting mitochondrial content mixing. Finally, we investigated the mechanistic basis for the observation that the wave drives mitochondrial motility in metaphase but mitochondrial fission in interphase. Our data indicate that when the force of actin polymerization is resisted by mitochondrial tethering to microtubules, fission results. In striking contrast, upon microtubule depolymerization actin wave-enveloped mitochondria in interphase cells display comet tail motility characteristic of metaphase cells, which are devoid of microtubule-mitochondria interactions, suggesting that microtubule tethering inhibits comet tail-driven motility.
HostingRepository	PRIDE
AnnounceDate	2024-06-23
AnnouncementXML	Submission_2024-06-23_01:16:21.562.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hossein Fazelinia
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-23 07:12:19	ID requested
⏵ 1	2024-06-23 01:16:22	announced

10.1038/s41467-024-48189-1;

Coscia SM, Moore AS, Thompson CP, Tirrito CF, Ostap EM, Holzbaur ELF, An interphase actin wave promotes mitochondrial content mixing and organelle homeostasis. Nat Commun, 15(1):3793(2024) [pubmed]

submitter keyword: FMNL1, actin,mitochondrial fission, homeostasis

Erika L.F. Holzbaur, Ph.D.
contact affiliation	Department of Physiology University of Pennsylvania Perelman School of Medicine 635 Clinical Research Building 415 Curie Boulevard Philadelphia, PA 19104-6058
contact email	holzbaur@pennmedicine.upenn.edu
lab head
Hossein Fazelinia
contact affiliation	Children's Hospital of Philadelphia
contact email	fazeliniah@email.chop.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD050085

PRIDE project URI

• PRIDE
  1. PXD050085
     1. Label: PRIDE project
     2. Name: An interphase actin wave promotes mitochondrial content mixing and 2 organelle homeostasis