PXD050063 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A Bioinspired Immunostimulatory System for Inducing Powerful Antitumor Immune Function by Directly Causing Plasma Membrane Rupture |
Description | Preparation of AMDs. 125 μM Ac4ManNAz were incubated with Raw 264.7 (~ 107 cells/dish) for 24, 48, and 72 h, followed by gentle washing with PBS or complete medium to produce Raw 264.7-Ac4ManNAz. Subsequently, Raw 264.7-Ac4ManNAz (~ 107 cells) were resuspended in PBS containing Alkynyl-Ce6(0.1 μM), ascorbic acid (0.2 μM) and CuSO4 (0.2 μM) and incubated for 1h at 37 °C. Then centrifuged (1000 rpm, 3 min) and washed 3 times with PBS to obtain precursor of AMDs. precursor of AMDs (~ 107 cells/tube, 1.0 ml) was immersed in liquid nitrogen, 24 h later, the frozen tubes were thawed at 37°C, centrifuged at 1500 rpm for 3 min, washed and resuspended by PBS to generate the AMDs (5 x 106 units/tube, 1.0 ml). |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-08 |
AnnouncementXML | Submission_2024-10-08_11:36:33.527.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | hao yin |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q-Tof Ultima |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-02-22 23:15:22 | ID requested | |
⏵ 1 | 2024-10-08 11:36:33 | announced | |
2 | 2024-10-22 06:34:19 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Mouse, Antitumor Immune, Plasma Membrane Rupture |
Contact List
hao yin |
contact affiliation | Institute for Advanced Research, Wenzhou Medical University, Wenzhou, Zhejiang, P.R. China. |
contact email | yinhao@wmu.edu.cn |
lab head | |
hao yin |
contact affiliation | Institute for Advanced Research, Wenzhou Medical University, Wenzhou, Zhejiang, P.R. China. |
contact email | yinhao@wmu.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD050063
- Label: PRIDE project
- Name: A Bioinspired Immunostimulatory System for Inducing Powerful Antitumor Immune Function by Directly Causing Plasma Membrane Rupture